Lab

Question 0

Inoculating loop should be in bacti-cinerator for a minimum of how many seconds?

Answer 0

5

Question 1

T-soy agar

Answer 1

• tryptic soy agar
• solid @ room temp
• allows for cultivation of many different microbes

Question 2

Bacti-cinerator

Answer 2

• no scalpels, forceps, or any sharp objects inside allowed

Question 3

Culture medium/media

Answer 3

The nutrient material prepared for growth of microorganisms in a lab

Question 4

Inoculum

Answer 4

Microbes introduced into a culture medium to initiate growth

Question 5

Culture

Answer 5

Microorganisms that grow & multiply in a container of culture medium

Question 6

Sterile medium

Answer 6

a medium that is free of organisms

Question 7

Agar

Answer 7

A complex polysacch derived from a marine alga & used as a solidifying agent in culture media

Question 8

Agar liquifies and solidifies at what temps, respectively

Answer 8

100˚C

40˚C

Question 9

Chemically defined media

Answer 9

A culture medium in which the exact chemical composition is known

Question 10

Fastidious organisms

Answer 10

Any organism that has a complex nutritional requirement. It will only grow when specific nutrients are included in its diet.

Question 11

Complex media

Answer 11

A culture medium in which the exact chemical composition is not known

Question 12

Nutrient broth

Answer 12

A complex medium made of beef extract & peptone

Question 13

Nutrient agar

Answer 13

Nutrient broth containing agar

Question 14

Selective media

Answer 14

A culture medium designed to suppress the growth of unwanted microorganisms & encourage the growth of desired

Question 15

Differential media

Answer 15

A solid culture medium that makes it easier to distinguish ones colonies of the desired organism

Question 16

Enrichment media

Answer 16

A media that promotes the growth of a particular organism by providing it w/the essential nutrients & rarely contains certain inhibitory substance to prevent growth of normal competitors