Lab 2 (Microscope) Flashcards

1
Q

Simple microscope

A

Single lens

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2
Q

Compound Microscope

A

series of lenses

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3
Q

Head

A

upper part of the frame, supports lenses, also called body tube

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4
Q

Arm

A

vertical part of the frame, connects head and base

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5
Q

Light switch

A

turns light source on/off

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6
Q

Rheostat

A

adjust intensity of light/light intensity knob

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7
Q

Light source

A

illuminates the specimen

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8
Q

Base

A

contains light source/bottom part of the frame

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9
Q

Ocular lenses

A

10x magnification/eyepieces

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10
Q

revolving nosepiece

A

supports objective lenses/allows changing of them

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11
Q

objective lenses

A

creates upside down image/4x/10x/40x/100x

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12
Q

4x magnification

A

scanning lens

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13
Q

10x magnification

A

high power lens

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14
Q

40x magnification

A

high power lens

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15
Q

100x magnification

A

oil immersion lens

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16
Q

side clips

A

holds slide in place on stage

17
Q

mechanical stage

A

platform for slide to sit on

18
Q

condenser

A

lens below the stage/concentrates light on specimen

19
Q

mechanical stage adjustor knob

A

moves stage anterior, posterior, sinister, and dexter

20
Q

fine focus knob

A

precision focusing

21
Q

Ocular lens power

22
Q

coarse focus knob

A

raises or lowers the stage

23
Q

Iris diaphragm

A

adjust contrast/regulates light reaching condenser

24
Q

How to carry the microscope

A

one hand on arm, one on base

25
lens paper
material used to clean ocular lenses
26
coverslip
for wet mount slide preperations
27
use the coarse adjustment knob ONLY with the ____
lowest-power lens (10x)
28
use the fine adjustment knob with _____
high power lenses
29
total magnification
power of ocular lens x power of objective lens
30
parfocal lenses
require little or no fine adjustment moving from one objective lens to the other
31
resolution of human eye
0.14mm (140um)
32
resolution of light microscope
0.0002mm (0.2um)
33
field of view
area of the object visible through the microscope
34
working distance
distance front lens of objective and surface of cover glass
35
high power
lens closest (smallest working distance) 400x total mag smallest FOV largest image
36
low power
lens medium distance (working distance) 100x total mag medium FOV medium image size
37
Scanning power
farthest working distance 40x total mag largest FOV smallest image
38
stage micrometer
1mm scale divided into 100 divisions (0.01mm/10um) used to calibrate ocular micrometer
39
Ocular micrometer
needs to be calibrated with stage micrometer at each magnification