Lab 2 (Microscope)

Question 1

Simple microscope

Answer 1

Single lens

Question 2

Compound Microscope

Answer 2

series of lenses

Question 3

Head

Answer 3

upper part of the frame, supports lenses, also called body tube

Question 4

Arm

Answer 4

vertical part of the frame, connects head and base

Question 5

Light switch

Answer 5

turns light source on/off

Question 6

Rheostat

Answer 6

adjust intensity of light/light intensity knob

Question 7

Light source

Answer 7

illuminates the specimen

Question 8

Base

Answer 8

contains light source/bottom part of the frame

Question 9

Ocular lenses

Answer 9

10x magnification/eyepieces

Question 10

revolving nosepiece

Answer 10

supports objective lenses/allows changing of them

Question 11

objective lenses

Answer 11

creates upside down image/4x/10x/40x/100x

Question 12

4x magnification

Answer 12

scanning lens

Question 13

10x magnification

Answer 13

high power lens

Question 14

40x magnification

Answer 14

high power lens

Question 15

100x magnification

Answer 15

oil immersion lens

Question 16

side clips

Answer 16

holds slide in place on stage

Question 17

mechanical stage

Answer 17

platform for slide to sit on

Question 18

condenser

Answer 18

lens below the stage/concentrates light on specimen

Question 19

mechanical stage adjustor knob

Answer 19

moves stage anterior, posterior, sinister, and dexter

Question 20

fine focus knob

Answer 20

precision focusing

Question 21

Ocular lens power

Answer 21

10x

Question 22

coarse focus knob

Answer 22

raises or lowers the stage

Question 23

Iris diaphragm

Answer 23

adjust contrast/regulates light reaching condenser

Question 24

How to carry the microscope

Answer 24

one hand on arm, one on base

Question 25

lens paper

Answer 25

material used to clean ocular lenses

Question 26

coverslip

Answer 26

for wet mount slide preperations

Question 27

use the coarse adjustment knob ONLY with the ____

Answer 27

lowest-power lens (10x)

Question 28

use the fine adjustment knob with _____

Answer 28

high power lenses

Question 29

total magnification

Answer 29

power of ocular lens x power of objective lens

Question 30

parfocal lenses

Answer 30

require little or no fine adjustment moving from one objective lens to the other

Question 31

resolution of human eye

Answer 31

0.14mm (140um)

Question 32

resolution of light microscope

Answer 32

0.0002mm (0.2um)

Question 33

field of view

Answer 33

area of the object visible through the microscope

Question 34

working distance

Answer 34

distance front lens of objective and surface of cover glass

Question 35

high power

Answer 35

lens closest (smallest working distance)
400x total mag
smallest FOV
largest image

Question 36

low power

Answer 36

lens medium distance (working distance)
100x total mag
medium FOV
medium image size

Question 37

Scanning power

Answer 37

farthest working distance
40x total mag
largest FOV
smallest image

Question 38

stage micrometer

Answer 38

1mm scale divided into 100 divisions (0.01mm/10um)

used to calibrate ocular micrometer

Question 39

Ocular micrometer

Answer 39

needs to be calibrated with stage micrometer at each magnification