LAB 2 Flashcards
Multicellular Organism
Multicellular organisms are organisms that consist of more than one cell, in contrast to unicellular organisms.
Osmosis
a process by which molecules of a solvent tend to pass through a semipermeable membrane from a less concentrated solution into a more concentrated one, thus equalizing the concentrations on each side of the membrane.
Difference between a multicellular organism and a colonial organism
The difference between a multicellular organism and a colonial organism is that individual one-celled organisms from a colony can, if separated, survive on their own, while cells from a multicellular life-form (e.g., cells from a brain) cannot.
What are microscopes used for
To study cells
What kind of microscope did we use?
Light microscope/compound microscope
Procedure A: Preparing slides of eukaryan single-celled and colonial protists
- Obtained samples of two organisms
- Prepared them on a wet mount slide
- Do not examine at 100%, since oil immersion lenses are necessary for that level of magnification
Look at pg. 19, and know the difference between a single cell organism, and colonial organism…easy
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Procedure B: Simple Staining of an Animal and Plant Cell
Used a cheek cell bc cheek cells from the epithelial tissue that lines the mouth of humans and other mammals is a good representation of a generalized animal cell
Epithelial tissue
Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces.
Steps for Procedure B: Human Cheek Cells
- scrap cheek with toothpick
- place 1-2 drops of TOLUIDINE blue stain on the slide
- rub the tip of the toothpick in the stain on the glass slide in order to transfer the cells to the slide and apply a COVERSLIP.
- examine the prepared cells with the compound microscope
- locate the nucleus
Steps for Procedure B: Onion Epidermal Cells
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Epidermal cell
The epidermis cells (from the Greek “επίδερμίδα”, meaning “over-skin”) is a single-layered group of cells that covers plants’ leaves, flowers, roots and stems. It forms a boundary between the plant and the external environment.
Two types of electron microscopes:
Transmission electron microscopes (TEM), and Scanning Electron Microscope (SEM)
TEM good for?
produces two-dimensional images, and is useful for viewing details of organelles such as the endoplasmic reticulum and the nucleus
SEM good for?
provides three-dimensional viewing