Lab 2

Question 1

how do you convert M into μM?

Answer 1

multiply by 1 000 000

Question 2

what is the slope and the y- intercept in the y=mx+b formula?

Answer 2

m= slope
b= y- intercept

Question 3

how would you find the concentration of the solution using the slope= 0.0033, y-int= 0.0376 and the absorbance of 0.053?

Answer 3

(0.053)=(0.0033)x-0.0376
x= 27.2µM

Question 4

the absorbance of each solution in this experiment was measured at 535nm. what was the colour of the beam in the spectrophotometer?

Answer 4

green this is because green is the absorbed color at 535nm on the visible light spectrum

Question 5

why do we need a blank measurement?

Answer 5

so that the background measurements are not read, therefore, it will ensure that the spectrophotometer will give solely the compound of interest for the other absorbance measurements

Question 6

what is normally used as a blank?

Answer 6

distilled water

Question 7

what color will the solution be with a wavelength of 730nm?

Answer 7

blue

Question 8

what color will a solution be with a wavelength of 500nm?

Answer 8

red

Question 9

what is a blank? what is normally used as a blank?

Answer 9

distilled water is normally used as the blank measurement.
we measure a blank so that the background measurements are not read, therefore, it will ensure that the spectrophotometer will give solely the compound of interest for the other absorbance measurements