Lab #2

Question 1

What is the name of SDS-PAGE

Answer 1

SDS-Polyacrylamide Gel Electrophoresis

Question 2

What is proteome

Answer 2

A complete set of porteins produce in a given cell, organism or tissue

Question 3

What does it mean when an SDS-PAGE gels are discontinuous?

Answer 3

most have two gels that are vertically stacked on top of each other

Question 4

What is the name of the top gel on an SDS-PAGE

Answer 4

Stacking gel

Question 5

What is the percentage of acrylamide and the Ph.H level of a stacking gel

Answer 5

4% and 6.8

Question 6

What happens when a protein enters a stacking gel

Answer 6

Regardless of size, the move very quickly and all end up in the resolving gel at the same time

Question 7

What is the name of the second gel in the SDS-PAGE

Answer 7

Resolving Gel

Question 8

What is the percentage of arylamide and the P.H level of a resolving gel

Answer 8

8-15% and 8.8

Question 9

How to the protein react once they enter the resolving gel

Answer 9

They move very slowly, but smaller proteins tend to reach the lower protion of the gel much faster than large proteins

Question 10

What is polyacrylamide gel made of?

Answer 10

Formed by polymerization of arylamide monomers into long linear chains

Question 11

Also known as the free radical generator

Answer 11

Ammonium persulfate

Question 12

Also kown as an organic catalyst

Answer 12

TEMED(tetramethylenediamine)

Question 13

methylene-bis-acrylamide

Answer 13

(bis for short) also included in the mixture

Question 14

Higher concentration of both bis and acrylamide leads to what

Answer 14

stiffer gel with small poers

Question 15

Lower concentration of both bis and acrylamide leads to what

Answer 15

softer gel with large pores

Question 16

What is electophoresed alonside “uknown” proteins

Answer 16

markers

Question 17

What is Relative mobility(RF)

Answer 17

the ratio of distance traveled by a given protein divided by the distance traveled by the tracking dye in the lane during that time

Question 18

Rf is plotted as what function of

Answer 18

base 10 logarithm(log10)