Lab #15

Question 1

BAP

Answer 1

Enriched - with 5% sheep blood; Differential - for type of hemolysis

Question 2

MSA

Answer 2

Selective - with 7.5% salt - for salt tolerant bacteria (G+); Differential - Mannitol fermentation - indicator - Phenol Red - pH <7 is yellow

Question 3

EMB

Answer 3

Selective - with Eosin and Methylene Blue - for G- bacteria; Differential - Lactose fermentation - indicator – Eosin and Methylene Blue – lots of acid accumulated = Green Metallic Sheen

Question 4

MAC

Answer 4

Selective - bile salts and crystal violet - for G- bacteria; Differential - Lactose fermentation - Indicator - Neutral Red - pH < 7 is pink precipitate

Question 5

PEA

Answer 5

Selective - Phenylethanol tolerance - for G+ bacteria