lab

Question 1

What is the most common approach to seperating complex mixtures of biological molecules

Answer 1

Chromatograohy

Question 2

Why is chromatography useful

Answer 2

To study a dingle type of protein or other molecules

Question 3

The extract contining the biomolecule we are looking at is called

Answer 3

mobile phase

Question 4

What passes over the stationary phase

Answer 4

The mobile phase

Question 5

How any forms of chromapathy are there? ad what are they?

Answer 5

Thin-layer and Column

Question 6

What happens to the stationary phase in a column chromatography

Answer 6

its compased of small beads

Question 7

What is chromatographic medium

Answer 7

small beads in a sraonary phase of a column chromatography

Question 8

What is the driving force of seperation in the column chromatography

Answer 8

Interaction with the mobile phase with chromatographic

Question 9

Interaction can be based on

Answer 9

1. Net charge(ion exchange chromatography)
2. Hydropobicity
3. Molecular size
4. Molecular relations like antigen-antiobody or enzyme-substrate

Question 10

Anything smaller than the pores size will

Answer 10

enter the pores and travel through passages in the interior of the beads

Question 11

Anything larger than the pores size

Answer 11

won’t be allowed to enter

Question 12

What is bed volume

Answer 12

A space where beads are packed within a column

Question 13

What is voild volume

Answer 13

The space between beads in a column bed

Question 14

Seperation of the molecules is deteremined by what

Answer 14

ability to enter the pores in the chromatographic medium

Question 15

Exclusion limit

Answer 15

Average pore diamter