Lab 10: RNA Sequencing

Question 1

What is the difference between the genome and the transcriptome?

Answer 1

The genome is the complete set of DNA in a cell. (DNA or RNA)
Transcriptiome is a a way for scientists to view a collection of transcribed RNA (mRNA) in a single point in time. (Only RNA)

Question 2

What are the main steps involved in RNA-seq? The ones we talked about (don’t google this or you will get many, many more steps).

Answer 2

**Main steps for RNA-seq: **
-in-vitro: Transcription(collect samples)
-in-vilico : Fragemnetation - mRNA makes reveresed DNA known as cDNA. (prepare and sequence RNA)
-in-silico: Sequence Processing - using computational methods scientists can make sense of the reads. they are able to identify the expression of each gene. Higher the amount of reads higher the expression of the gene. (analyze the data).

Question 3

What is bioinformatics? How is it important to biology?

Answer 3

Bioinformatics is biologists using computer science to answer qeustions about Biology.
Its important for Biology because through this we’re able to whoch genes are highy expressed

Question 4

What are the two columns in the HT-seq files? What do the “reads” represent?

Answer 4

The two collumns of the HT-seq files are the gen ID and read count.
The reads represent the