Lab 1: ubiquity testing, aseptic technique, and bacterial isolation techniques

Question 1

What are the two ways of making microorganisms visible?

Answer 1

1. microscopy in conjugation with staining

2. culturing

Question 2

_________: when a mass of genetically identical bacteria is produced through the multiplication of a single cell

Answer 2

binary fission

Question 3

______: a group of microbial cells growing on a solid surface that is visible to the naked eye

Answer 3

a colony

Question 4

the cells that make up a colony are therefore considered to be genetically ____ and _________

Answer 4

pure and identical

Question 5

The material that microbiologists use to grow microorganisms is called __________

Answer 5

medium

Question 6

__________ microbes have special nutrient requirements or differentiate one group of microbes from another

Answer 6

fastidious

Question 7

Liquid media is called ______

Answer 7

broth

Question 8

Solid media is called ________

Answer 8

agar

Question 9

________: a thick layer on the surface of a broth

Answer 9

pellicle

Question 10

________: particles that have settled to the bottom of a broth

Answer 10

sediment

Question 11

solid media is then referred to as _________ _________

Answer 11

nutrient agar

Question 12

does agar supply nutrients to microbes?

Answer 12

no

Question 13

__________: microbes are put into the agar when it is semi liquid but not so hot that it kills them

Answer 13

pour-plate technique

Question 14

______ _______: are used when we want a large number of cells for use in a single application

Answer 14

broth tubes

Question 15

a test tube with nutrient agar is called _____ ______ and it is most useful for growing microbes with low or zero O2 requirements deeply within the agar

Answer 15

agar deep

Question 16

An ______ ______ is a test tube of nutrient agar that was allowed to solidify at an angle creating a larger flat surface for growth at the top of the agar

Answer 16

agar slant

Question 17

An _______ ______ is a petri plate containing nutrient agar that provides the largest surface area fro growth of microorganisms

Answer 17

agar plate

Question 18

_____ cultures: contain only one species of microbe

Answer 18

pure

Question 19

_______ ________: sequential transferring a constant volume minimum of three times that results in the decrease of concentration

Answer 19

serial dilution

Question 20

how do we calculate the number of viable cells (colony forming units in the original sample)

Answer 20

of colonies on the plate X dilution factor / mL of dilution plated