Lab 1: Sanitation Flashcards
Who are some key people responsible for inventing sanitation protocols
Ignaz Semmelweis
Florence nightingale
What are two events/organizations created in 1980 to enforce sanitation
FDA Mandate
CDC:Center for Disease control
Why is washing ones hands important for us in the lab?
We need to wash our hands to protect ourselves and avoid transmitting bacteria and viruses to one another, lab surfaces and food that we will be handling.
What are some examples of places of where you need to wash your hands
Hospital
food service areas such as restaurants, farm fields and placed where food is packaged and shipped to other locations
places where people are closely clumped together such as cruise ships and schools
What are the situations in which you need to wash your hands
After being sick, handling tissues and blowing your nose
After using the restroom
Are all bacteria on your skin bad? What about commensal bacteria?
No there are 10,000 to 10,000,000 CFU/cm^2 bacteria on your hands which are not bad. There are 1000 different species on your skin. These include dermabacteria, staphlococcus epidermis, coreyebacterium, enterobacter, micrococcus, and pseduomonas.
What are some examples of pathogens that can infect you?
Resident:
Transient:
Resident pathogens (already in your body or can become apart of your body on some occasions)
1) Staphlococcus aureus
2) Kleibisella, Pseudomonas (infrequently)
What are some examples of pathogens that can infect you?
Resident:
Transient:
Resident pathogens (already in your body or can become apart of your body on some occasions)
1) Staphlococcus aureus (traditionally and always)
2) Kleibisella, Pseudomonas (infrequently)
Transient:
1)Norovirus
2)E.coli
3)Salmonella
What are some of the materials used in lab?
1) Tryptic soy agar
2) -Hand sanitizing solution:
- Shop brand hand sanitizer
- Alcholic sanitizer
- Soap
Describe the procedure for lab
The purpose of this lab was to visualize bacteria on your hands before and after you have touched different surfaces, and examine the efficiacy of different sanitizing solutions. Two petri dishes were removed and divided into two columns one where a D was marked for dirty and the other where an S was marked for sanitized. Lab members placed their thumbs on the D surface for 30 seconds and sanitized it with a particular solution and then did the same thing for the S column. On a seperate petri dish, lab members placed their thumbs against a solid surface and then touched one of the columns on the petri dish for 10 seconds, washed their thumb and placed it on the column labeled dirty for 10 seconds. The bacteria for both plates were visualized afterwards.
