LAB 1 - MICROSCOPY AND THE CELL

Question 1

Turns the lamp on and off

Answer 1

Power switch

Question 2

Contains a set of lenses that focus the light on the specimen.

Answer 2

Condenser

Question 2

• Consists of a lamp bulb and one or more lenses to produce a cylindrical beam of light directed toward the base of the condenser
• May have ground glass: a frosted sheet of glass that scatters light from the lamp bulb to provide more diffuse light.
• May have an iris diaphragm called the field diaphragm which controls the diameter of the illuminating beam of light and used as a guide to focus the light from the condenser onto the specimen.

Answer 2

Illuminator

Question 2

Controls the brightness of the lamp

Answer 2

Illumination control

Question 3

Moves the condenser up and down to adjust the focus of the light on the specimen

Answer 3

Focusing knob

Question 4

Controls the aperture of the illuminating light and used to adjust contrast

Answer 4

Iris diaphragm

Question 4

The glass surface closest to the specimen. Be careful not to touch this surface as it is easily scratched

Answer 4

Front lens

Question 5

Holds several objective lenses that can be rotated into position to change the lens

Answer 5

Revolving nosepiece

Question 6

Create a magnified image of the specimen

Answer 6

Objective lenses

Question 7

Ued to get an overview of the structures present in a section and to find areas for more detailed observation

Answer 7

Scanning lens

Question 8

The most useful magnification to identify tissues

Answer 8

LPO 10x lens

Question 9

Used to see the details of cell and tissue organizatio

Answer 9

HPO 40x lens

Question 10

Because it requires the use of immersion oil, is used primarily to see subcellular details.

Answer 10

OIO 100x lens

Question 11

Forms an image that can be visualized by the eye or a camera.

Answer 11

Eyepiece

Question 12

Raise and lower the specimen stage to focus the image of the specimen

Answer 12

Focusing controls

Question 13

Focus the specimen at 4x and 10x

Answer 13

Coarse focus

Question 14

Focus the specimen at 40x and 100x, but only after initially focusing at lower magnification

Answer 14

Fine focus

Question 15

Spring-loaded device to hold the microscope slide in place on the stage

Answer 15

Slide holder

Question 16

Ability to enlarge an image

Answer 16

Magnification

Question 17

Allow the slide to be moved along two axes: longitudinal and lateral

Answer 17

Slide holder travel controls

Question 18

Holds the microscope slide

Answer 18

Specimen Stage