Lab 1 Flashcards
Cytology
Study of cells
Histology
Study of tissues
Gross Anatomy
Structures large enough to see with the naked eye
Light Microscope
- Also called Optical Microscope
- Uses visible light and lenses to magnify an object
Simple
Single lens
Compound
Light passes through a series of lenses
How do you carry a microscope correctly?
Grasp the arm with one hand and rest the microscope’s base on other hand
Head
- Upper part of frame
- Supports lenses
- Also called body tube
Arm
- Vertical part of frame
- Connects head and base
Light Switch
-Turns light source off and on
Rheostat
- Adjusts intensity of light
- Also called the light intensity knob
Base
- Bottom part of frame
- Contains light source
Light Source
- Found within the base
- Illuminates the specimen
Slide Clips
- hold slide in place on stage
Mechanical stage
- platform for slide to sit on
Mechanical stage adjustor knob
- moves stage forward, back, left, or right
Fine focus knob
- used for precision focusing
Coarse focus knob
- raises or lowers the stage
Iris diaphragm
- adjusts contrast
- opens and closes to regulate the amount of light reaching the condenser
Condenser
- lens below the stage
- concentrates light onto the specimen
Objective lenses
- four strengths
- scanning lens, 4x magnification
- low power lens, 10x magnification
- high power lens, 40x magnification
- oil immersion, 100x magnification
High Power
- lens closest to slide
- 400x total magnification
- smallest field of view
- largest image
Low Power
- lens medium distance from slide
- 100x total magnification
- intermediate size field of view
- intermediate size image
Scanning Power
- lens farthest from slide
- 40x total magnification
- largest field of view
- smallest image
Revolving nosepiece
- supports objective lenses
- rotates to change objective lenses
What are all living things composed of?
Cells
What are 3 common characteristics of cells?
- The outer boundary of cell is the plasma membrane.
- All cells store genetic information in the form of DNA.
- Everything inside the plasma membrane that is not DNA or nucleus is cytoplasm.
What is the plasma membrane?
The outer boundary of the cell.
What form do cells store genetic information in?
DNA
What is inside the plasma membrane that is not DNA or the nucleus?
Cytoplasm
What is the levels of organization in hierarchical manner?
- Organelles
- Cells
- Tissues
- Organs
- Systems
(OC TOS)
What are Organelles?
- Structures found inside a cell
- Each performs a function necessary for a cells to survive and perform its duties
- Example: nucleus containing the DNA of the cell
What are Cells?
- Composed of organelles
- Most basic unit of life because a cell divides to reproduce itself
- Example: cardiac muscle cell
What are tissues?
- composed of identical cells
- four basic types in the body
- each type performs a specific function
- example: cardiac muscle tissue
What are organs?
- composed of two or more types of tissue
- these work together to perform a specific function
- example: the heart contract to pump blood
What are systems?
- group of organs with related functions
- each system carries out one of the primary functions necessary to sustain life
- example: the cardiovascular system carries blood throughout the body to deliver vital substances like oxygen
Nucleus
- contains DNA
- pattern for building all proteins for the cell
Nuclear envelope
- membrane around nucleus
- DNA can’t cross it
Nucleolus
- area in nucleus
- site of rRNA synthesis
Chromatin
- unwound DNA inside nucleus
Nuclear pores
- opening in nuclear envelope
- allows mRNA out of nucleus
Cytoskeleton
- several different types
- internal scaffolding for cell
Cytoplasm
- semi-solid substance
- fills space between plasma membrane and nucleus
- composed of organelles suspended in cytosol
Plasma membrane
- outer boundary of cell
- lipid bilayer
- regulates what enters or leaves cell
Ribosomes
- composed of rRNA and proteins
- site of mRNA translation
- free ribosomes are in cytoplasm
- fixed ribosomes stud outside of RER
Rough endoplasmic reticulum (RER)
- membranous organelle
- network of channels
- site of protein synthesis
Mitochondrion
- membranous organelle
- bean shaped
- site of ATP synthesis
Lysosome
- membrane bound vesicle
- contains hydrolytic enzyme
Smooth endoplasmic reticulum (SER)
- membranous organelle
- forms tube like structure
- site of lipid synthesis
Centriole
- paired organelle
- organizes spindle fibers during mitosis
Golgi apparatus
- membranous organelle
- forms stack of plates
- proteins packaged for secretion
Transport vesicle
- membrane bound vesicle
- transports substances throughout the cell
Interphase
- consists of G1, S, & G2 phases
- time during which cell is carrying out its functions and growing larger
- replicates DNA as it prepares to divide
First Growth (G1) phase
- cell grows lager
- carries out cellular functions
Synthesis (S) phase
- DNA is replicated
- sister chromatids are formed
- organelles increase in number
Second Growth (G2) phase
- cells grows larger
- prepares to divide
- continues to carry out cellular functions
Cell division
- consists of the four stages of mitosis and cytokinesis
- time during which original cell produces two identical daughter cells
Mitosis
- equal division of DNA
- consists of four stages: prophase, metaphase, anaphase, and telophase
Cytokinesis
- division of cytoplasm
- results in two equal cells
Interphase
- cell carries out its functions
- DNA is diffuse and called chromatin
- no chromosomes are visible
- cell replicates DNA and organelles as it prepares to enter mitosis