Lab 1 Flashcards
Microscope
instrument and tool used in the study of cells, tissues, and small organisms
Compound Microscope
most commonly used microscope that’s composed of a lens system, light source, and adjusting mechanisms
Stereo Microscope
microscope used to look at larger or opaque objects
Base
rests on table and contains a built-in light source and light switch
Light Source
part of microscope where light comes from
Arm
rises from base and supports stage, lens system, and control mechanisms
Stage
flat surface where microscope is placed
Stage Clips/Mechanical Stage & Control
Stage clips/mechanical stage holds slide in place and stage control allows movement of slide
Condenser
located below stage and concentrates light on object being observed
Iris Diaphragm
built within the condenser and enables you to control the amount of light entering the lens system
Ocular lens
eyepiece
Nosepiece
part that has attached objective lenses
Scanning Objective Lens
4x magnification
Low Power Objective Lens
10x magnification
High Dry Objective Lens
40x magnification
Oil-immersion Objective Lens
100x magnification
Course Focus Knob
brings objects into rough focus when using the 4x and 10x objectives.
Fine Focus Knob
used to bring objects into sharp focus; used with 40x and 100x objectives
Magnification
ratio of image size to actual size
Total Magnification
calculated by multiplying ocular (10x) by the objective lens.
Resolving Power/Resolution/Resolve
ability of a lens or microscope to distinguish between two closely adjacent points or two separate objects
Contrast
difference in luminance and/or color that makes an object distinguishable; improved by reduction in light;
Parfocal
meaning if an object is centered and in sharp focus with one objective, it will be the same with another objective
Wet Mount Slide
slide with specimen mounted in a liquid (water) and covered with a glass
Field of View/Field
circle of light that can be seen; amount of object you can see will decrease with increasing magnification
Depth of Field
Vertical distance within which structures are in sharp focus; decreases as magnification increases
Diameter of Field
helps in estimating the size of the objective
Working Distance
Distance from the end of the objective to the specimen; decreases with increasing magnification