L9-11: Yeast and NanoBiotechnology, Cancer Flashcards
Statin drug Simvastatin causes loss of _______
mitochondrial DNA
The nanoscale (nanoscopic dimensions) covers sizes between ____ and ____.
100nm and 1.0 nm
In nanoscale, a material’s properties depend on its __________.
size, shape and composition
T or F:
A nanoparticle’s properties differ significantly to its properties in the bulk.
True.
How to confirm nanoparticle/quantum dot is inside micelle
Electron microscopy using lipid staining
How to confirm DNA is sitting on the nanoparticle
Use complementary DNA labeled with fluorescent agarose beads
Biodegradable nanocapsules for DNA encasing and delivery (sacrificial template method)
Positively charged MnCO3 microparticle (sacrificial) is encased by layers of negatively charged DNA through electrostatic attraction. The DNA is then encased by a polymer. In an acid solution, the sacrificial template (MnCO3) will dissolve, leaving behind a hollow cylinder. Enzymes can then liberate and digest the DNA for gene delivery.
Multifunctional carriers
Use of sacrificial template to encase quantum dots and magnetic particles. Upon sacrifice of template (e.g. dissolving in acid) only the magnetic particles will be left and can be separated using magnet.
Components of a molecular biosenser
- Molecular recognition element (e.g. enzyme, Ab, receptors etc.) which will create a biological interaction response
- Signal transducer - converts biological response to a quantifiable signal (electrochemical, optical etc.)
Example of electrical detection of DNA hybridisation
- Target DNA is immobilised on a nitrocellulose membrane
- Ag nanoparticle DNA probe is hybridised to the DNA
- DNA-bound nanoparticles are submerged in an acidic solution and are oxidatively released.
- Dissolved Ag nanoparticles can then be detected through ASV
