L8 Flashcards by A E

true or false, TEs are extremely abundant in eukaryote genomes

true

How well did you know this?

Not at all

Perfectly

true or false, most TE are relics and incapable of movement

true

How well did you know this?

Not at all

Perfectly

What is the Ac element

discovered by McClintock, standard non replicative DNA TE

causes mutability by excision from gene
Ac DNA can jump from place to place

How well did you know this?

Not at all

Perfectly

true or false, Ac element causes mutability by excision from gene

true

How well did you know this?

Not at all

Perfectly

true or false, Ac DNA can jump from place to place

true

How well did you know this?

Not at all

Perfectly

true or false, Ac element blocks transcripting and splicing

true

How well did you know this?

Not at all

Perfectly

true or false, Ac transposase conservatively

true

How well did you know this?

Not at all

Perfectly

true or false, excision of Ac is imprecise and leaves insertions 6-8 bp

true

How well did you know this?

Not at all

Perfectly

true or false, Ac insertions in coding region are usually inactive, non mutable

true

How well did you know this?

Not at all

Perfectly

true or false, classical mutable alleles are caused by insertion into introns or promoters

true

How well did you know this?

Not at all

Perfectly

what is Ds

dissociator element (site of chromosomal breaks); non autonamous

How well did you know this?

Not at all

Perfectly

what is Ac

activator element (activation of dissociator); autonamous, can hop by itself

How well did you know this?

Not at all

Perfectly

true or false, Both Ds and Ac can transpose, but Ds needs Ac in the same cell to do so

true

How well did you know this?

Not at all

Perfectly

why is Ds non autonamous

lack active transposase

How well did you know this?

Not at all

Perfectly

true or false, Ds elements are simple deletion mutants of Ac, contain the same terminal repeats as Ac

true

How well did you know this?

Not at all

Perfectly

what is the P element in Drosophila

standard DNA TE

true or false, P element target germ line cells

true

how large is the human genome

3.2 Gb

human genome total protein coding region number of genes

21000 genes

how many TEs in human genome

3 M (45%)

what are the major classes of TE repeats

DNA TE
retroviral
LINE
SINE

what are the RNA transposable elements

retroviral
LINE
SINE

size retroviral

5-10 kb

size of LINE

6-8 kb

size of SINE

100-300 bp

true or false, there is a strong correlation between TE content and genome size

true

name a yeast transposable element

Ty1

how many DR does Ty1 make at insertion sites

5 bp

what are LINEs

- long interspersed elements - abundant in mammalian genomes (100 000 copies) - encode a single protein with both RT activity and inegrase activity - transpose via RNA - no terminal repeats

what are SINES

- short interspersed elements - transpose via RNA - extremely abundant in mammalian genome - 300 bp long, do not code for genes, and are probably derived from cellular RNAs involved in splicing - SINEs are therefore non autonamous- appear to use the LINE protein for transposition - no terminal repeats - generate short DRs when they integrate

true or false, most eukaryote TEs move infrequently

true

true or false, only 9000 transpostion events in total in humans then

true

true or false, individuals differ at 285 loci per genome

true

why are TEs conserved

allowed rearrangement of DNA