L5 synthetic biology Flashcards
1
Q
sigma factor
A
- needed for transcription
- different for different genes
2
Q
the equation for degradation of mRNA over time
A
dYm/dt = beta(m) - alpha(m) x Ym
- beta = production of mRNA
- alpha = degradation of mRNA
3
Q
what influences beta(m)?
A
- concentration of DNA
- K for RNAPs promoter
- concentration of sigma factor which depends on temperature
- concentration of RNAP
- concentration of repressor
- concentration of inducer
4
Q
what influences alpha(m)?
A
- concentration of RNAse
- growth rate
5
Q
what happens at steady state?
A
- dYm/dt = 0
- Ym(ss) = beta(m)/alpha(m)
6
Q
koff/kon?
A
= K(RNAP) = [RNAP]x[P]/[RNAP-P]
7
Q
dissociation constant
A
= R = 1/Keq
8
Q
R* and beta(m)
A
- if R* = 0 => beta(m) = beta(max)
- if R* = huge => beta(m) = 0
- if R* = K(R) => beta(m) = beta(max)/2
9
Q
equation with Yp
A
- don’t measure Ym, measure Yp because proportionate/similar to Ym
dYp/dt = beta(p) - alpha(p) x Yp
10
Q
genetic circuits
A
- like electrical circuit
-> RNAP like electrons - want to use transcription factors
-> activator or repressor
11
Q
what does Yp depend on?
A
- Ym
- K(ribosome)
- mRNA
12
Q
how to control amount of protein?
A
- 2 control knobs = TF and RBS
13
Q
transferable component for control
A
there’s a component containing gene for LacI with its’ promoter, TSS, terminator and operator. If this is put in front of a gene the LacI will block the gene from being transcribed
-> put in front of gene you want to control
14
Q
hybrid promoters
A
- ex. Ara-Lac
- has 2 operators
15
Q
promoter with 2 inputs
A
- some natural promoters need 2 inputs
-> 1st = sugar
-> 2nd = cAMP
