L32

Question 1

ER transport involves

Answer 1

Signal sequence
SRP complex - binds to receptor in ER membrane
Translation continues and translocation begins
SRP displaced and recycled

Question 2

Signal sequence is

Answer 2

Hydrophobic
A start-transfer signal
Within the peptide sequence, not NH2 terminal

Question 3

Signal peptidase :

Answer 3

Cleaves off signal sequence

Question 4

“Stop Transfer” sequence :

Answer 4

Hydrophobic
Causes polypeptide to stop in translocator
Resistant to peptidase

Question 5

Vesicle transport budding

Key players :

Answer 5

Adaptin, clathrin, cargo receptor, dynamin

Question 6

Adaptin binds :

Answer 6

Clathrin and cargo receptor

Question 7

Dynamin is :

Answer 7

A GTPase
Destabilises neck bud thus pinching off occurs
Requires energy from hydrolisation

Question 8

What comes off in budding?

Answer 8

Clathrin and adaptin, only cargo receptor remains

Question 9

Vesicle targeting

Key players :

Answer 9

Rab, Rab effector, SNARE (t and v), GDI

Question 10

Rab is :

Answer 10

A small GTPase

Guides vesicle

Question 11

Rab effector is :

Answer 11

Binds to RabGTP to guide it

Question 12

Where is the vSNARE?

Answer 12

At the vesicle-containing-cargo

Question 13

What’s the trans-SNARE-complex?

Answer 13

A mix of tSNARE and vSNARE that helps fusion by bringing membrane close to each other

Question 14

Identify the 3 coat proteins involved between ER and Golgi

Answer 14

COP 1 (between cisternae, involved in cargo transport in cisternae), COP 2(between ER to Golgi), clathrin (recycling and Golgi to somewhere else like plasma membrane)

Question 15

2 mechanisms of cisternae transport?

Answer 15

Cisternae maturation, vesicle transport between cisternae