L3: Vibrio, Campylobacter, Helicobacter (E Mokaddas)

Question 1

What are the characteristics and morphology of Vibrio Cholerae

Answer 1

Question 2

How can you differentiate between V.cholerae and V.parahemolyticus + V.vulnificus?

Answer 2

V.cholerae:
~Non-halophilic vibrios (only in freshwater)
~Does not have a vector; transmitted by man
~non-capsulated

V.parahemolyticus + V.vulnificus:
~Halophilic vibrios (like salt water)
~Have a vector; can be transmitted by shellfish (food poisoning)
~ V.vulnificus contains polysaccharide capsule

Question 3

Where would you find V. Cholerae

Answer 3

Question 4

What type of media does V. Cholerae grow on?

Answer 4

Question 5

Answer 5

Question 6

What is the pathogenesis of V. Cholerae

Answer 6

Question 7

What are the clnical features of V. Cholerae

Answer 7

Question 8

What is TCBS media?

Answer 8

Question 9

How is each vibrio species shown on TCBS agar?

Answer 9

V. Cholerae:
Sucrose fermenter - yellow

V.parahemolyticus & V. Vulnificus:
NON sucrose fermenters - green

Question 10

Which vibrio species causes Acute Gastroenteritis?

Answer 10

V.parahemolyticus

Question 11

What type of diarrhea does each vibrio species cause?

Answer 11

~V. Cholerae:
Severe diarrhea (and bloody diarrhea in V.Cholerae non-01)

~V.parahemolyticus:
Explosive diarrhea

~V. vulnificus:
Acute diarrhoea following consumption of shell fish

Question 12

What are the clinical features of V.parahemolyticus?

Answer 12

Question 13

What are the 3 main clinical syndromes of V.vulnificus?

Answer 13

Question 14

What is the commonest cause of infective diarrhoea in developed countries ?

Answer 14

Campylobacter jejuni and coli

Question 15

What is the morphology of C.Jejuni and C.coli?

Answer 15

Question 16

C.Jejuni and C.coli Pathogenesis:

Answer 16

Question 17

C.Jejuni and C.coli location:

Answer 17

Question 18

Clinical features of C.Jejuni and C.coli?

Answer 18

Question 19

C.Jejuni and C.coli Laboratory diagnosis

Answer 19

Question 20

What do Campylobacter and Heliobacter have in common?

Answer 20

~ oxidase +
~ motile
~ gram negative spiral shaped bacilli

Question 21

Heliobacter Pylori description:

Answer 21

Question 22

Here is Helicobacter pylori located?

Answer 22

ONLY in gastric mucosa (stomach)

Not colon

Question 23

How is H. Pylori transmitted?

Answer 23

Oral-oral or fecal-oral rout

Question 24

What is the pathogenesis for H.pylori

Answer 24

Question 25

What is the duration of H.pylori?

Answer 25

Incubation period: few days

Lasts for: 2 weeks

Question 26

Helicobacter pylori Laboratory diagnosis:

Answer 26

Question 27

Clinical manifestations of H.pylori

Answer 27