L3 - Herbicide Tolerance Flashcards

1
Q

define weed

A

plant growing in the wrong place at the wrong time

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2
Q

Weeds contribute to what losses

A

loss in production 13.2% $76bn

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3
Q

What quantitative damage do weeds cause

A

due to competition with water, light and nutrients, and to antagonism (parasitism and allelopathy)

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4
Q

What qualitative damage do weeds cause

A

indirect damage due to crop yield reduction, contamination of seeds, slowing of tillage and harvesting practices, and degradation of quality of milk or other animal products

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5
Q

How can weeds be controlled

A

• Herbicides: but heavy usage has lead to groundwater contamination, death of wildlife species, human/animal illnesses

(Also farmers have to use both broad-spectrum herbicides (before planting) and several narrow-spectrum herbicides (during crop growth))

  • Tillage: leaves valuable topsoil exposed to wind and water erosion
  • Hand weeding: Very labour intensive, not suitable for large scale
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6
Q

Herbicides contribute how much of the pesticide market

A

45%

$33bn

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7
Q

N. America has the greatest sales of pesticides T/F

A

T - 27% of the market

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8
Q

Whats the most common herbicide, whats its LD50 value

A

Glyphosate
LD50 301 for humans - low mammalian toxicity

(lower the value the more toxic)

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9
Q

What mode of action is taken on grasses and dicots, Roundup is an example of this mode.

A

•EPSPS inhibitors (grasses & dicots): The enzyme EPSPS is used in the synthesis of the amino acids tryptophan, phenylalanine & tyrosine.
Glyphosate (Roundup) is a systemic EPSPS inhibitor inactivated by soil contact

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10
Q

What are ACCase inhibitors

A

•ACCase inhibitors (kill grasses): Acetyl coenzyme A carboxylase (ACCase) is part of the first step of lipid synthesis. Thus, ACCase inhibitors affect cell membrane production in the plant meristems

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11
Q

What are ALS inhibitors

A

•ALS inhibitors (grasses & dicots): The acetolactate synthase (ALS) enzyme is the first step in the synthesis of the branched-chain amino acids (valine, leucine, isoleucine) eventually leading to inhibition of protein synthesis

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12
Q

How do synthetic auxins work?

A

mimic this plant hormone. They have several points of action on the cell membrane, and are effective in the control of dicot plants. E.g. 2, 4-D

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13
Q

Discuss Photosystem II inhibitors mode of action

A

reduce e- flow from water to NADPH2+ at the photochemical step in photosynthesis. Cause electrons to accumulate on chlorophyl molecules -> increased oxidation -> death

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14
Q

Discuss Photosystem I inhibitors mode of action

A

steal e- from the normal pathway through FeS – Fdx – NADP leading to direct discharge of electrons on oxygen

  • > reactive oxygen species
  • > high levels of oxidation
  • > plant death
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15
Q

List all the modes of action - herbicides

A
  1. EPSPS inhibitors
  2. ACCase inhibitors
  3. ALS inhibitors
  4. Synthetic auxins
  5. Photosystem II inhibitors
  6. Photosystem I inhibitors
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16
Q

When was roundup made by who/when/what is it?

A

Monsanto
1970
Glyphosate - EPSPS inhibitor

17
Q

What reaction to EPSP synthase? How does ESPSS inhibitors work?

A

S3P + phosphenolpyruvate –> ESP

Glyphosate binds to phosphenolpyruvate the enzyme cannot bind

18
Q

Herbicides can be categorised into what two groups? discuss them

What is the AIM

A
  • Selective herbicides kill weeds but not the crop plant: can be sprayed on a growing crop
  • Non-selective herbicides are only usable before the crop emerges
  • Easier to develop non-selective herbicides than selective ones

• Aim: make crop plant resistant to a non-selective herbicide. Herbicide will then kill weeds but not the crop plant: herbicide is made to be selective

19
Q

What are the 4 mechanisms of herbicide resistance/tolerance

A
  1. Over-expression of protein which is the target for the herbicide
  2. Alteration of the site of herbicide action
  3. Introduction by gene transfer techniques of herbicide detoxification genes from bacteria
  4. Herbicide detoxification in plants
20
Q

What gene found were encodes glyphosate resistance? how?

A

aroA gene
S. typhimurium/ E. coli
Makes glyphosate less able to bind to target protein “Alteration of the site of herbicide action”

21
Q

psbA encodes resistance to what?

A

Attrazine

22
Q

Glufosinate resistance is encoded by what gene? found where? what type of gene is thi?

A

bar gene
streptomyces
detoxification gene from bacteria

23
Q

Give an example of Herbicide detoxification in plants

A

glutathione-S-transferase (GST). This enzyme occurs in plants and renders xenobiotics ineffective by conjugating them to reduced glutathione

24
Q

How does Soybean 40-3-2 work?

A

•Resistant to glyphosate (Roundup)
•Contains a mutant form of EPSPS (from CP4 strain of agrobacterium tumefaciens) which does not bind to glyphosate, therefore conferring resistance
- Herbicide tolerance

25
Q

How does glufosinate work and what is it?

A

Glufosinate (an alternative to Glyphosate)

  • Glufosinate is an active ingredient in several nonselective systemic herbicides e.g. Basta, Rely, Finale, Ignite, Challenge, and Liberty
  • It interferes with the biosynthetic pathway of the amino acid glutamine and with ammonia detoxification
26
Q

Advantages of herbicide tolerance crops

A
  • Excellent weed control and hence higher crop yields
  • Flexibility – possible to control weeds later in the plant’s growth
  • Reduced numbers of sprays in a season
  • Reduced fuel use (because of less spraying)
  • Reduced soil compaction (because of less need to go on the land to spray)
  • Use of low toxicity compounds which do not remain active in the soil
  • The ability to use no-till or conservation-till systems, with consequent benefits to soil structure and organisms
27
Q

Glyphosate is degraded to what in the soil?

A

AMPA –> Co2 by soil microbes

residues move max 6inches in soil, stong adsorption, low risk of moving to group waters

28
Q

What are the risks associated with herbicide tolerance crops?

A
  1. • Persistence or invasiveness of crops
  2. • Increase in glyphosate usage
  3. • Resistance to glyphosate in weeds
29
Q

What is a more durable strategy of ht crops?

A

engineer with different modes of transport to minimise risks of resistance to glyphosate in weeds