L2- Cell cycle mitosis, their regulation Flashcards
Cell cycle
prokaryotic cells- cell cycle occurs via a process termed binary fission.
eukaryotic cells, the cell cycle can be divided in two periods- a) interphase b) mitosis
Interphase
G1 phase:- Growing Synthesize new proteins and organelles Doing their jobs Longest phase of cell cycle
S Phase
Chromosomes(DNA) are replicated
Key proteins associated with replication are made (centromeres)
G2 Phase
Shortest of the 3 phases of interphase
Organelles and molecules for cell division are produced (centrioles)
Check-up phase before mitosis.
Checkpoints
A checkpoint in the cell cycle is a critical control point where stop and go signals regulate the cycle.
cell cycle regulators
Protein kinases
Internal Regulators
Cdk
MPF
p53
p27
Cdk
Adds phosphate to a protein
major control switches for the cell cycle
to move the cell from g1 to s and s to g2
MPF
Cdk and cyclin that triggers progression through the cell cycle.
p53
protein that blocks the cell cycle if dna is damaged
p27
protein that binds to cyclin and cdk to block entry into s phase.
Endogenous hormones
Auxin, cytokinins, abscisic acid, gibberellins and brassinosteroids.
Interphase
chromosomes are extended form and seen as chromatin.
Nucleus is visible
Prophase
nucleus and nuclear envelope break down
chromatin condenses into chromosomes
spindle fibres grow from centrioles
centrioles migrate to opposite poles
Metaphase
chromosomes align at the center
spindle fibers are attached
nuclear membrane has disappeared
Anaphase
centromere split and sister chromatid are pulled to poles
Telophase
chromosomes again become longer and thin
new nuclear membrane formed
cell division almost complete