L12 TCA cycle, ET chain, Oxidative phosphorylation Flashcards
net gain from glycolysis and link reaction?
2 ATP
4 NADH
2 ACoA
pyruvate dehydrogenase deficiency?
- sex linked
- build up of life threatening lactic acid (lactic acidosis)
- symptoms appear shortly after birth - most don’t survive past childhood
- neurological problems/delayed development
why is there a build up of lactic acid in pyruvate dehydrogenase deficiency?
- pyruvate can not be converted to ACoA , NAD+ regenerated by reduction of pyruvate (= lactic acid)
why do neurological problems occur with pyruvate dehydrogenase deficiency?
TCA cycle can not proceed = energy deficit = congenital brain malformation
where does the citric acid cycle occur?
matrix of mitochondria
arsenic poisoning?
- headaches, confusion, severe diarrhoea and drowsiness
- convulsions and changes in fingernail pigmentation (leukonychia striata)
- arsenic interferes with cellular longevity by allosteric inhibition of pyruvate dehydrogenase.
Which enzyme does arsenic poisoning inhibit?
pyruvate dehydrogenase
which substrate in the TCA cycle is not coupled with the production of NADH?
Succinate
what accumulates in pyruvate dehydrogenase deficiency?
lactic acid
how many CO2 molecules does the krebs cycle produce?
4
which enzyme in krebs allows for substrate level phosphorylation?
succinyl co-A synthase
which enzymes in krebs reduce NAD+?
Isocitrate dehydrogenase
alpha-ketoglutarate dehydrogenase
Malate dehydrogenase
where does the ET chain occur?
intergral membrane proteins in inner mitochondrial membrane
what reactions occur when electrons are passed through the chain?
redox - exergonic reactions
what is the final electron acceptor in ETC?
oxygen to form water
