L11 Microscopy

Question 1

What are bright field/light microscopes used to look at?

Answer 1

Bacteria
Fungi
Parasites

Question 2

What is fluorescence microscopy used to look at?

Answer 2

Bacteria
Fungi
Parasites
Viruses

Question 3

What are dark field microscopy used to look at?

Answer 3

Spirochetes

Question 4

What is Electron microscopy used to look at?

Answer 4

Viruses and Microsporidia

Question 5

What color do gram positive and gram negative bacteria show?

Answer 5

Gram Positive: Purple

Gram Neg: Pink

Question 6

What are gram stains useful for?

Answer 6

Direct examination for dx
Determine quality of specimen
Bacterial ID
Clinical relevance of culture

Question 7

What art stains?e acid fas

Answer 7

Used to stain bacteria with cell walls with long chain fatty acids

uses heat/detergent to drive stain into cell

Ex: Mycobacteria

Question 8

What are special fungal stains?

Answer 8

Lactophenol Cototn Blue
Gomor Methenamine SIliver
Periodic Acid Schiff
Mucicarmine

Question 9

What silver stains are used for bacteria and spirochetes?

Answer 9

Warthin Starry

Steiner

Question 10

What are the parasite stains?

Answer 10

Wright’s Giemsa
Wet Mounts
Trichrome
Modified Acid Fast

Question 11

What are the differentmethods for fluorescent microscopy?

Answer 11

Auramine-Rhodamine: mycobacteria
Calcofluor White: Fungi
Fluorescein Conjugated Abs: Virus