Knowledge

Question 1

MWC cooperativity model

Answer 1

• Cooperative proteins have several identical units (protomers)
• Each protomer has one binding site and these binding sites are all equivalent
• The protomer has two conformational states, R and T, which differ in their ability to bind ligand
• If the binding of a ligand to one site causes a conformational change between R and T then the same
change is induced in all the other protomers (‘concerted’ change)

Question 2

Explain lac operon, with cAMP

Answer 2

-

Question 3

Explain trp

Answer 3

-

Question 4

3 method of studying metabolic networks

Answer 4

kinetic models
flux based approaches
characterising global properties

Question 5

Observation advantages and disadvantages

Answer 5

ad: real?
dis: quality inv-prop to coverage, expensive

Question 6

Inference advantages and disadvantages

Answer 6

ad: high coverage, cheap
dis: assumptions, need high-quality obs, noisy, error prone, conditional relationships

Question 7

Generations of functional analysis techniques

Answer 7

Over representation analysis
Functional class scoring
Topology based approaches