Know your cytokines

Question 1

B7

Answer 1

On: APCs
Funct.: 2nd sig. of act. for T-Cells
Pairing: CD28 on T-Cells

Question 2

CD-2

Answer 2

On: TCells
Funct.: adhesion molecule
Pairing: APC or Target cell

Question 3

CD28

Answer 3

On: TCell
Funct.: Binds B7 as 2nd sig. of Act. TCells
Pairing: B7 on APCs

Question 4

CD3 complex

Answer 4

On: Tcell
Funct.: Moves alpha and beta subunits of TCR to cell surface and does signal transduction
Pairing: TCR

Question 5

CD34

Answer 5

On: HEV of 2nd Lymph.
Funct.: Binds to L-Selectin for adhesion
Pairing: L-Selectin

Question 6

CD40

Answer 6

On: B-Cell
Funct.: Survival Sig. for aff. mat.
Pairing: CD40 Ligand on T-helper

Question 7

CD40 ligand

Answer 7

On: T Helper Cells
Funct.: Triggers Affinity Maturation
Pairing: CD40

Question 8

Chemokine

Answer 8

On: HEV 2nd Lymph Tiss.
Funct.: activates LFA-1
Pairing: LFA-1

Question 9

CR2

Answer 9

On: B-Cell
Funct.: Co-Receptor on Bcells, binds comp. fragments
Pairing: Breakdown products of C3b

Question 10

DC-CK (Dendritic Cell Cytokine)

Answer 10

On: Dendritic Cells
Funct.: attracts T cells
Pairing: T Cells

Question 11

Fas Ligand

Answer 11

On: NK Cells + Act. T Cells
Funct.: Binding Fas triggers apoptosis
Pairing: Fas

Question 12

Fc-gammaRIII

Answer 12

On: NK Cell Surface
Funct.: Binds IgG bound to infected host cells
Pairing: IgG Fc Region

Question 13

Fc-epsilonRI

Answer 13

On: Mast Cells, Eosinophils
Funct.: Binds IgE
Pairing: Fc portion of IgE

Question 14

FoxP3

Answer 14

By: Treg cells

Funct.: Regulates T Cell differentiation. May also initiate differentiation to Treg Cells

Question 15

GlyCAM-1

Answer 15

On: HEV on 2nd Lymph. Tissues
Funct.: Diapedesis/entry of naive T Cell into 2nd lymph.
Pairing: L-Selectin

Question 16

ICAM-1

Answer 16

On: APC or Target Cell
Funct.: Adhesion molecule
Pairing: LFA-1

Question 17

Ig-alpha/Ig-beta complex

Answer 17

On: B Cell
Funct.: Signal transduction unit for B Cells
Pairing: BCR

Question 18

LFA-1

Answer 18

On: Effector T Cell
Funct.: Adhesion Molecule, diapedesis
Pairing: ICAM-1 on APC

Question 19

L-selectin

Answer 19

On: Naive T Cells
Funct.: Diapedesis/entry of naive T Cells to 2nd Lymph
Pairing: addressins: CD34 and GlyCAM-1

Question 20

PECAM-1

Answer 20

On: endothelial Cells
Funct.: Modulates transmigration
Pairing:

Question 21

VCAM-1

Answer 21

On: Vascular epithelium
Funct.: binds to VLA-4
Pairing: VLA-4

Question 22

VLA-4

Answer 22

On: effector T Cell
Funct.: Binds Vascular Epithelium, entry to site of inflammation
Pairing: VCAM-1

Question 23

IFN-gamma

Answer 23

Secreted by: Th1 Effector cells, CD8 T Killers
Function: Signals Th0 cells to become Th1 cells; upregulates macrophage activity; activates acute phase response; induces iron storage, inhibits erythropoietin prod.

Question 24

IFN-alpha and beta

Answer 24

Secreted by: Virus-infected host cells

Funct.: upregulates MHC-I on adjacent cells; activates NK cells

Question 25

GM-CSF

Answer 25

Secreted by: NK Cells | Funct.: Modulates macrophage activity

Question 26

TNF-alpha

Answer 26

Secreted by: Macrophages | Funct.: Activates acute phase response; Causes endothelial cell retraction and leakage. Induces ICAM-1 and IFN-gamma

Question 27

TNF-beta

Answer 27

Secreted by: macrophages | Funct.: causes endothelial cell leakage and induces ICAM and Ig Superfamily

Question 28

TGF-beta

Answer 28

Secreted by: macrophages | Funct.: Fibroblast migration and proliferation, monocyte chemotaxis; induces VEGF production

Question 29

EGF

Answer 29

Secreted by: Macrophages | Funct.: Fibroblast migration and proliferation

Question 30

FGF

Answer 30

Secreted by: macrophages | Funct.: fibroblast migration and proliferation, monocyte chemotaxis, promotes collagen synthesis

Question 31

PDGF

Answer 31

Secreted by: Macrophages | Funct.: fibroblast migration and proliferation, monocyte chemotaxis, promotes collagen synthesis

Question 32

VEGF

Answer 32

Secreted by: hypoxic cells | Funct.: Angiogenesis

Question 33

IL-1

Answer 33

Secreted by: Macrophages, and NK Cells Funct.: Activates acute phase reaction, responsible for inducing fever, endothelial cell retraction, modulates macrophage activity, induces expression of ICAM and other Ig Superfamily members

Question 34

IL-2

Answer 34

Secreted by: T-Cells | Funct.: Reqd. for proliferation of T-Cells

Question 35

IL-3 (Loc/Func.)

Answer 35

Location: Bone Marrow | Funct.: Induces macrophage differentiation

Question 36

IL-4 (Loc/Func./Sec. By)

Answer 36

Secreted by: Th2 CD4 Cells Funct.: Signals Th0 to become Th2, activates B cells, promotes class switching to IgE Loc.: germinal center

Question 37

IL-5 (Loc/Func./Sec. By)

Answer 37

Secreted by: Th2 CD4 cells Funct.: Signals Th0 to become Th2, activates B cells, signal B lymphoblasts to plasma cells, promotes class switching to IgA Loc.: medullary cords of 2nd lymph, germinal cent.

Question 38

IL-6 (Loc./Func./Sec. By)

Answer 38

Secreted by: Macrophages, Th2 CD4 cells Funct.: Act. acute phase response, induces CRP, Th0 ->Th2, Act. B Cells, B-Cells->Plasma Cells Loc.: Medullary cords of 2nd lymph, germinal center

Question 39

IL-7 (func.)

Answer 39

Func.: survival of memory T-Cell and developing B-Cells

Question 40

IL-8 (Func.)

Answer 40

Func.: chemical gradient for chemotaxis, stimulates release of histamine

Question 41

IL-10

Answer 41

Secreted by: macrophages | Funct.: anti-inflammatory

Question 42

IL-12

Answer 42

Secreted by: macrophages | Funct.: Signals Th0 -> Th1, induces IFN-gamma prod. in T Cells and NK cells.

Question 43

Credits: Thanks to Robert Hester for putting the information for this packet together. It wouldn't have been possible without you.

Answer 43

Credits: Thanks to Robert Hester for putting the information for this packet together. It wouldn't have been possible without you.