Klebsiella

Question 1

Klebsiella species are found in which habitat??

Answer 1

They are commonly found in human intestines and as a saprophytes in soil

Question 2

Genus klebsiella has two species which are??

Answer 2

They are K.pneumoniae and K.oxycota

Question 3

Similar to E.coli,Klebsiella species are also lactose fermenter however they differ by??

Answer 3

In being non-motile and capsulated (possess capsular polysaccharide)

Question 4

K.pneumoniae has a three subspecies which are ??

Answer 4

1. subspecies pneumoniae
2. subspecies ozaenae
3. subspecies rhinoscleromatis

Question 5

Subspecies pneumoniae (most pathogenic)are responsible for??

Answer 5

Severe lobar pneumonia,urinary tract infections, meningitis (neonates),septicaemia and pyogenic infections such as abscesses and wound infections

Question 6

Subspecies pneumoniae colonises??

Answer 6

Colonizes the oropharynx of hospitalised patients and is a common cause of nosocomial infections.most of the hospital strains are multidrug resistant .

Question 7

Pneumonia present with??

Answer 7

Pneumonia tends to be destructive with production of thick , mucoid, ,brick red sputum.sometime, the sputum has a thick jelly-like appearance.
-some strains can rarely cause diarrhea and have been shown to produce an E.coli like heat stable enterotoxin.

Question 8

Subspecies ozaenae is associated with??

Answer 8

It is associated with atrophic rhinitis(or ozena) characterised by foul smelling nasal discharge. It is biochemically inactive.

Question 9

(LAB DIAGNOSIS) biochemical property

K.pneumoniae gram staining show ??

Answer 9

Klebsiella is a short ,plump, straight capsulated gram-negative rods, about 1-2micro metre *0.5-0.8 micro metre in size .

Question 10

K.pneumoniae on culture MacConkey agar it produces:

Answer 10

It produces large dome shaped mucoid (due to capsule ) sickey , pink colour, lactose fermenting colonies

Question 11

(BIOCHEMICAL IDENTIFICATION)
ICUT
Indole test:

Answer 11

Negative

Question 12

Citrate test:

Answer 12

Positive (citrate is utilised)

Question 13

Urease test :

Answer 13

Positive (urea is hydrolysed)

Question 14

TSI(triple sugar iron agar test):

Answer 14

Shows acid/acid, gas present,H2S absent

Question 15

Sugar fermentation test:

Answer 15

Ferments most of the sugars such as glucose, lactose, mannitol, maltose (but not sucrose) with production of acid and gas

Question 16

VP(Voges-Proskauer) test:

Answer 16

Positive ( detect the acetoin production) bacteria convert glucose to acetoin

Question 17

MR(methyl red)test:

Answer 17

Negative

Question 18

K.oxytoca is biochemically similar to K.pneumoniae but differs in??

Answer 18

K.oxytoca are indole positive