KK 2 DNA manipulation

Question 1

function of polymerases

Answer 1

enzymes that synthesise nucleic acids

Question 2

function of ligases

Answer 2

a group of enzymes that join fragments of DNA or RNA in a process called ligation

Question 3

Function of endonucleases

Answer 3

restriction enzymes that cut DNA into smaller and more useable fragments

Question 4

what does CRISPR-Cas9 stand for

Answer 4

Cluster regularly interspaced short palindromic repeats - CRISPR-associated protein 9

Question 5

what is CRISPR-Cas9

Answer 5

an acquired immune system for bacteria, short repeated segments of DNA each separated by a spacer

Question 6

PAM sequence

Answer 6

a nucleotide triplet with two repeating guanine bases (NGG)