KIA, Gelatin, Urea, Lysine PPT Flashcards by Kimberly Castillo

Q

Glucose/lactose fermentation test media?

A

KIA

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Q

Glucose/lactose enzyme?

A

none

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Q

Glucose lactose test pH indicator?

A

phenol red

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Q

Glucose/lactose test reagent?

A

none

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Q

Glucose/lactose test end product?

A

Acid & gas

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Q

Glucose/lactose test (+) result ?

A

glucose only ferment: red slant yellow butt
lactose and glucose: yellow slant and butt
displacement/cracks : (+) gas

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Q

glucose/lactose (-) test?

A

no change: no fermentation

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Q

H2S production test media?

A

KIA

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Q

H2S Test enzyme ?

A

Sodium thiosulfate reductase

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Q

H2S Test pH indicator ?

A

none

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Q

H2S Test reagent?

A

iron salt

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Q

H2S end product?

A

H2S hydrogen sulfide

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Q

H2S (+) result?

A

black precipitate

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Q

H2s (-) result?

A

no change : no black pecipitate

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Q

Gelatin test media?

A

gelatin strip

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Q

Gelatin test enzyme?

A

gelatinase

Q

Gelatin test pH indicator ?

A

none

Q

Gelatin Test reagent?

A

none

Q

Gelatin test end product?

A

liquification

Q

Gelatin Test (+) result ?

A

liquification: strip blue

Q

Gelatin test (-) result?

A

no change strip green

Q

Urea hydrolysis test media?

A

urea broth

Q

urea hydrolysis test enzyme

A

urease

Q

urea hydrolysis test pH indicator

A

phenol red

urea hydrolysis test reagent ?

none

urea hydrolysis end product?

ammonia

urea hydrolysis (+) result?

pink color

urea hydrolysis (-) result?

yellow/ no change

Lysine decarboxylase test media

lysine decarboxylase broth

lysine decarboxylase enzyme?

decarboxylase

lysine decarboxylase pH indicator

bromocresol purple

Lysine decarboxylase reagent?

mineral oil

lysine decarboxylase end product?

Co2 | cadaverine

lysine decarboxylase (+) result?

purple color

Lysine decarboxylase (-)

yellow/ no change