KIA, Gelatin, Urea, Lysine PPT Flashcards

1
Q

Glucose/lactose fermentation test media?

A

KIA

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2
Q

Glucose/lactose enzyme?

A

none

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3
Q

Glucose lactose test pH indicator?

A

phenol red

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4
Q

Glucose/lactose test reagent?

A

none

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5
Q

Glucose/lactose test end product?

A

Acid & gas

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6
Q

Glucose/lactose test (+) result ?

A

glucose only ferment: red slant yellow butt
lactose and glucose: yellow slant and butt
displacement/cracks : (+) gas

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7
Q

glucose/lactose (-) test?

A

no change: no fermentation

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8
Q

H2S production test media?

A

KIA

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9
Q

H2S Test enzyme ?

A

Sodium thiosulfate reductase

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10
Q

H2S Test pH indicator ?

A

none

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11
Q

H2S Test reagent?

A

iron salt

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12
Q

H2S end product?

A

H2S hydrogen sulfide

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13
Q

H2S (+) result?

A

black precipitate

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14
Q

H2s (-) result?

A

no change : no black pecipitate

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15
Q

Gelatin test media?

A

gelatin strip

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16
Q

Gelatin test enzyme?

A

gelatinase

17
Q

Gelatin test pH indicator ?

A

none

18
Q

Gelatin Test reagent?

A

none

19
Q

Gelatin test end product?

A

liquification

20
Q

Gelatin Test (+) result ?

A

liquification: strip blue

21
Q

Gelatin test (-) result?

A

no change strip green

22
Q

Urea hydrolysis test media?

A

urea broth

23
Q

urea hydrolysis test enzyme

A

urease

24
Q

urea hydrolysis test pH indicator

A

phenol red

25
Q

urea hydrolysis test reagent ?

A

none

26
Q

urea hydrolysis end product?

A

ammonia

27
Q

urea hydrolysis (+) result?

A

pink color

28
Q

urea hydrolysis (-) result?

A

yellow/ no change

29
Q

Lysine decarboxylase test media

A

lysine decarboxylase broth

30
Q

lysine decarboxylase enzyme?

A

decarboxylase

31
Q

lysine decarboxylase pH indicator

A

bromocresol purple

32
Q

Lysine decarboxylase reagent?

A

mineral oil

33
Q

lysine decarboxylase end product?

A

Co2

cadaverine

34
Q

lysine decarboxylase (+) result?

A

purple color

35
Q

Lysine decarboxylase (-)

A

yellow/ no change