KIA, Gelatin, Urea, Lysine PPT Flashcards
Glucose/lactose fermentation test media?
KIA
Glucose/lactose enzyme?
none
Glucose lactose test pH indicator?
phenol red
Glucose/lactose test reagent?
none
Glucose/lactose test end product?
Acid & gas
Glucose/lactose test (+) result ?
glucose only ferment: red slant yellow butt
lactose and glucose: yellow slant and butt
displacement/cracks : (+) gas
glucose/lactose (-) test?
no change: no fermentation
H2S production test media?
KIA
H2S Test enzyme ?
Sodium thiosulfate reductase
H2S Test pH indicator ?
none
H2S Test reagent?
iron salt
H2S end product?
H2S hydrogen sulfide
H2S (+) result?
black precipitate
H2s (-) result?
no change : no black pecipitate
Gelatin test media?
gelatin strip
Gelatin test enzyme?
gelatinase
Gelatin test pH indicator ?
none
Gelatin Test reagent?
none
Gelatin test end product?
liquification
Gelatin Test (+) result ?
liquification: strip blue
Gelatin test (-) result?
no change strip green
Urea hydrolysis test media?
urea broth
urea hydrolysis test enzyme
urease
urea hydrolysis test pH indicator
phenol red
urea hydrolysis test reagent ?
none
urea hydrolysis end product?
ammonia
urea hydrolysis (+) result?
pink color
urea hydrolysis (-) result?
yellow/ no change
Lysine decarboxylase test media
lysine decarboxylase broth
lysine decarboxylase enzyme?
decarboxylase
lysine decarboxylase pH indicator
bromocresol purple
Lysine decarboxylase reagent?
mineral oil
lysine decarboxylase end product?
Co2
cadaverine
lysine decarboxylase (+) result?
purple color
Lysine decarboxylase (-)
yellow/ no change