Key Question Answers From Exam Workbook Flashcards
How is DNA stored in a nucleus
Wound around Histone
Tightly coiled into chromosomes
Eukaryotic DNA
Not associated with proteins
Condensed by supercoiling
Complete set of proteins from a cell
Proteome
Prokaryotic DNA
No introns no non coding sequences
Shorter fewer repeat sequences between genes
Key words
Ribosomal RNA Catalysing Proteome Complimentary base pairing Adjacent tRNAs Peptide bond formation ATP Amino acid chain is extended Stop signal Codon RNA polymerase DNA helicase Template strand Exons Introns CDNA
Mitochondria DNA
Mitochondria DNA is shorter, circular, and is not associated with proteins.
Degenerate
Means multiple DNA codons can code for one amino acid.
Translation
The mRNA attaches to a ribosome.
TRNA molecules carry amino acid to the ribosome.
The TRNA is attached to the mRNA via complimentary base pairing.
The amino acid is on adjacent TRNA are joined together with peptide bonds.
ATP provides the energy for peptide bond formation.
The ribosome moves along the mRNA and the amino acid chain is extended.
The process continues until a stop signal on the mRNA is reached.
Translation
After the DNA has been unwound by DNA helicase.
RNA polymerase lines up free RNA nucleotides along the template strand.
According to the rules of complimentary base pairing.
RNA polymerase then joins the RNA nucleotides together as it moves along the DNA template strand.
Once RNA polymerase reaches a stop signal, it detaches and the mRNA is released.
CDNA
CDNA only contains exons
This is because cDNA is made from mRNA, which has been spliced so had its introns removed.