key info 2 for exams Flashcards

1
Q

Key events in transcription:
and diagram

A

https://cognitoedu.org/coursesubtopic/b3-alevel-aqa_sEoImuSp
-RNA polymerase enzyme binds to DNA.

  • hydrogen bonds between the DNA bases break, and the two strands of the double helix separate.

-The antisense strand acts as the template for mRNA synthesis.

-Free RNA nucleotides align with the DNA template through complementary base pairing.

-In the RNA molecule, uracil pairs with adenine from the DNA strand, while adenine from the RNA molecule pairs with thymine, and cytosine pairs with guanine.

-RNA polymerase catalyses the formation of phosphodiester bonds between adjacent RNA nucleotides.

  • A complementary mRNA strand is formed, carrying the same base sequence as the DNA sense strand.

-The process ends when RNA polymerase reaches a stop codon, detaches from DNA and terminates transcription.

-mRNA is released, detaches from DNA, and DNA rewinds into its double helix structure.

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2
Q

what type of organisms’ (eukaryotes or prokaryotes) mRNA undergoes splicing? and what is splicing

A

In eukaryotes, pre-mRNA is spliced after transcription to remove introns(non coding bases) before it is transported to the cytoplasm but splicing doesn’t occur in prokaryotes.

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