KA : 1.1 Structure of DNA Flashcards

1
Q

What is DNA?

A

DNA is inherited and is the genetic material of living things.

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2
Q

Where is DNA located?

A

DNA is located within the nucleus of all cells apart from red blood cells.

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3
Q

What is a genotype?

A

When a DNA sequence differs between individuals.

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4
Q

“A DNA molecule is comprised of __ chains of ______”

A

A DNA molecule is comprised of 2 chains of nucleotides.

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5
Q

What is a nucleotide made up of?

A

A phosphate, a deoxyribose sugar and any 1 of the 4 bases.

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6
Q

What are the 4 bases?

A

Adenine, Guanine, Cytosine and Thymine.

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7
Q

What is the base pairing rule?

A

Bses are said as being complementary to each other meaning there shapes match up.
Adenine → Thymine
Cytosine →Guanine

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8
Q

What are the bases joined by?

A

Hydrogen bonds

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9
Q

What forms the sugar phosphate backbone?

A

Nucleotides in a chain that are held together by strong chemical bonds between the phosphate and sugar make long chains of nucleotides called polynucleotides which form the sugar phosphate backbone.

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10
Q

Why is the 5’ and 3’ used?

A

The phosphate is attached to the carbon 5 of the deoxyribose sugar.
The base is attached to the carbon 1 of the deoxyribose sugar.

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11
Q

How is the double helix structure formed?

A

The chains of polynucleotides are joined together by the bases, by hydrogen bonds which is twisted round to create a double helix.

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12
Q

“The double helix is described as having __/_____/____ of nucleotides because one side goes from _’ to _’ and the opposite side goes from _’ to _’.”

A

The double helix is described as having two
anti-parallel chains of nucleotides because one side goes from 5’ to 3’ and the opposite side goes from 3’ to 5’.

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13
Q

What forms the genetic code?

A

The base sequence of DNA forms the genetic code.

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14
Q

What is meant by prokaryote?

A

A prokaryote is when cells lack a nucleus and most organelles.

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15
Q

What is meant by eukaryote?

A

A eukaryote is when cells have a nucleus and organelles.

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16
Q

DNA organisation in prokaryotes

A

Prokaryotes usually have a single circular chromosome and smaller circular plasmids.

17
Q

DNA organisation in eukaryotes

A

Eukaryotes all have linear chromosomes in the nucleus, which are tightly coiled and packaged with associated proteins. They also contain circular chromosomes in their mitochondria and chloroplasts.

18
Q

“DNA is tightly packaged with proteins to form a _______.”

A

DNA is tightly packaged with proteins to form a nucleoid.

19
Q

What is a special example of a eukaryote?

A

Yeast.

20
Q

Explain DNA packaging in eukaryotes

A
  1. DNA double helix is wrapped around histone proteins forming nucleosomes.(beads on a string)
  2. The chain of nucleosome then folds into a thicker chromatin fibre.
  3. The thick chromatin fibre then folds again, on a non-histone protein scaffold, to form looped fibres.
  4. The looped chromosome then folds further to produce a condensed chromosome.
21
Q

What are the pieces of DNA between nucleosomes known as?

A

Linker DNA.

22
Q

What are chromatin?

A

The combination of DNA and protein.