K. Factors That Affect Enzyme Activity Flashcards
pH
Denatures enzyme if too low or high
Temperature
Denatures enzyme if too high
Slows down enzyme if too low
Cofactors
Small, inorganic, usually metal ion
Coenzyme
Small, organic
Ex. Vitamins
Ex. N.A.D, F.A.D
Inhibitors
Decrease the enzymes function
Inhibitors:
Competitive inhibition
Attach to active site
Out competes the normal substrates for entrance
Inhibitors:
Non competitive inhibition
Bond to non active site location
Decrease enzyme activity because of changing its shape (denature)
Limiting factors
The factor (substance) in shortest supply will determine the rate of he reaction.
Limiting factors:
Enzyme concentration
Low amounts of enzyme concentration can limit the rate of the reaction
Limiting factors:
Substrate concentration
Low amounts of substrate concentration can limit the reaction
Limiting factors:
Saturation
Too busy
What are the factors that affect enzyme activity
- Temperature
- pH
- Cofactors
- Coenzymes
- Inhibitors
- Limiting factors