Journal 1 - Wnt-2 and Lung Cancer

Question 1

Wnt-2 Signaling

Answer 1

A molecular pathway where the Wnt-2 protein binds to Frizzled receptors, triggering a series of cellular events that influence cell growth and development

Question 2

Wnt-2

Answer 2

A protein that binds to Frizzled receptors to activate the Wnt signaling pathway, playing a key role in cell development and cancer progression

Question 3

Frizzled-8 (FZD8)

Answer 3

A receptor protein on the cell surface that binds to Wnt proteins, initiating the Wnt signaling pathway which is important for cell development and growth

Question 4

Beta-Catenin (β-catenin)

Answer 4

A protein involved in cell signaling and gene transcription, playing a crucial role in the Wnt signaling pathway and cell-cell adhesion

Question 5

Endogenous

Answer 5

Originating or produced within an organism, tissue, or cell.

Question 6

Exogenous

Answer 6

Originating or produced outside an organism, tissue, or cell.

Question 7

Constitutive Expression

Answer 7

Continuous expression of a gene, not regulated by external stimuli.

Question 8

TCF

Answer 8

T-cell factor, a transcription factor activated downstream of Wnt signaling, regulating target gene expression.

Question 9

Reporter Assay

Answer 9

An assay that measures the activity of a gene’s promoter, often used to study signal transduction pathways.

Question 10

RT-PCR (Reverse Transcription-PCR)

Answer 10

A technique to amplify and detect specific RNA sequences, used to study gene expression.

Question 12

Wnt-2 Signaling

Answer 12

A molecular pathway where the Wnt-2 protein binds to Frizzled receptors, triggering a series of cellular events that influence cell growth and development

Question 13

Wnt-2

Answer 13

A protein that binds to Frizzled receptors to activate the Wnt signaling pathway, playing a key role in cell development and cancer progression

Question 14

Frizzled-8 (FZD8)

Answer 14

A receptor protein on the cell surface that binds to Wnt proteins, initiating the Wnt signaling pathway which is important for cell development and growth

Question 15

Beta-Catenin (β-catenin)

Answer 15

A protein involved in cell signaling and gene transcription, playing a crucial role in the Wnt signaling pathway and cell-cell adhesion

Question 16

Endogenous

Answer 16

Originating or produced within an organism, tissue, or cell.

Question 17

Exogenous

Answer 17

Originating or produced outside an organism, tissue, or cell.

Question 18

Constitutive Expression

Answer 18

Continuous expression of a gene, not regulated by external stimuli.

Question 19

TCF

Answer 19

T-cell factor, a transcription factor activated downstream of Wnt signaling, regulating target gene expression.

Question 20

Reporter Assay

Answer 20

An assay that measures the activity of a gene’s promoter, often used to study signal transduction pathways.

Question 21

RT-PCR (Reverse Transcription-PCR)

Answer 21

A technique to amplify and detect specific RNA sequences, used to study gene expression.

Question 22

WNT Canonical Pathway

Answer 22

1. Wnt binds to frizzled
2. Wnt signaling is activated
3. DVL helps stabilize B-Catenin
4. B-catenin enters cell nucleus
5. B-catenin associates with (LEF/TCF) transcription factors
6. Promotes transcription of genes shown to cause cancer.

Question 23

Summary of Figure 1A

Answer 23

Wnt-2 is overexpressed in cancer, but what receptor is it expressed with? When tested in 293T cells to see which frizzled receptor could bind to Wnt-2 (and activate TCF factor) Frizzled-8 had the highest effective interaction with Wnt-2.

fzd-8 and wnt-2 activity super high together

Q: What is the relationship between Wnt-2 interactions with it’s receptors (frizzled) in lung cancer

Question 24

What method was used in Figure 1A?

Answer 24

TCF Reporter Assay - it detected activation of Wnt canonical pathway

assay that measures the activity of a gene’s promoter

Question 25

What conditions/treatments were evaluated in Figure 1A?

Answer 25

Used the presence of Wnt-2 to see if different Frizzled receptors would induce T cell factor (TCF)-dependent transcription.