jan synoptic Flashcards
What is the function of the Ribosomes
Site of Protein synthesis;
What is the function of the Nucleus
Contains genetic material / DNA;
Controls cell activity;
What is the function of the Mitochondria
Site of aerobic respiration;
ATP production;
What is the function of the Smooth Endoplasmic Reticulum
Site of lipid synthesis;
What is the function of the Rough Endoplasmic reticulum
Encrusted in Ribosomes;
Site of protein synthesis;
Transports and stores protein within the cell
What is the function of Golgi Apparatus
Modifies/packages/sorts proteins;
Produces vesicles;
What is the function of Lysosomes
Contains digestive enzymes;
Digests worn out organelles/autolysis;
What is the function of Cell surface membrane
Made of a Phospholipid Bi-layer;
Controls what enters the cell/ is selectively permeable;
Can be folded to increase SA;
What is the function of Chloroplasts
Contain thylakoids, stacked into Granum;
Site of photosynthesis;
What is the function of Capsule
What is the function of Capsule Protects cell from immune systems;
Aids bacteria sticking together;
What is the function of Plasmid
Circular DNA;
Contains antibiotic resistance genes;
What is the function of Cell Wall
Provides rigid shape / structure;
Stops osmotic lysis;
What is the function of Flagellum
Allows movement/propulsion;
Eukaryotic cells produce and release proteins. Outline the role of organelles in the production, transport and release of proteins from eukaryotic cells.
- DNA in nucleus is code (for protein);
- Ribosomes/rough endoplasmic reticulum produce (protein);
- Mitochondria produce ATP (for protein synthesis);
4 Golgi apparatus package/modify; OR Carbohydrate added/glycoprotein produced by Golgi apparatus;
5 Vesicles transport OR Rough endoplasmic reticulum transports; - (Vesicles) fuse with cell(-surface) membrane;
Describe the differences between Eukaryotic and Prokaryotic cells
comparisons:
1. Nucleotide structure is identical;
2. Nucleotides joined by phosphodiester bond;
OR Deoxyribose joined to phosphate (in sugar, phosphate backbone);
3. DNA in mitochondria / chloroplasts same / similar (structure) to DNA in prokaryotes;
Contrasts:
4. Eukaryotic DNA is longer;
5. Eukaryotic DNA contain introns, prokaryotic DNA does not;
6. Eukaryotic DNA is linear, prokaryotic DNA is circular;
7. Eukaryotic DNA is associated with / bound to protein / histones, prokaryotic DNA is not;
State three differences between DNA in the nucleus of a plant cell and DNA in a prokaryotic cell.
Plant v prokaryote
1. (Associated with) histones/proteins v no histones/proteins;
2. Linear v circular;
3. No plasmids v plasmids;
4. Introns v no introns;
5. Long(er) v short(er);
The structure of a cholera bacterium is different from the structure of an epithelial cell from the small intestine. Describe how the structure of a cholera bacterium is different
- Cholera bacterium is prokaryote;
- Does not have a nucleus/nuclear envelope/ has DNA free in cytoplasm/has loop of DNA;
3 and 4 Any two from: [No membrane-bound organelles/no mitochondria / no golgi/no endoplasmic reticulum];
5 Small ribosomes only;
6 and 7 Any two from [Capsule/flagellum/plasmid / cell wall]
Give one advantage of using a TEM rather than a SEM.
- Higher resolution;
- higher (maximum) magnification / higher detail (of image);
OR - Allows internal details / structures within (cells) to be seen / cross section to be taken;
Give one advantage of using a SEM rather than a TEM.
Thin sections do not need to be prepared / shows surface of specimen / can have 3-D images;
Scientists use optical microscopes and transmission electron microscopes to investigate cell structure. Explain the advantages and limitations of using a TEM to investigate cell structure.
Advantages:
1 Small objects can be seen;
2 TEM has high resolution;
3 Electron wavelength is shorter;
Limitations:
4 Cannot look at living cells;
5 Must be in a vacuum;
6 Must cut section / thin specimen;
7 Preparation may create artefact;
Scientists isolated mitochondria from liver cells. They broke the cells open in an ice-cold, buffered isotonic solution. Explain why the solution was:
a) Isotonic
b) Ice cold
c) buffered
a) Prevents osmosis / no (net) movement of water So organelle/named organelle does not burst/shrivel;
b) Reduce/prevent enzyme activity so organelles are not digested / damaged;
c) Maintain a constant pH so proteins do not denature;
Describe and explain how cell fractionation and centrifugation can be used to isolate mitochondria from a suspension of animal cells. (6)
- Cell homogenisation to break open cells and release organelles;
- Filter to remove (large) debris/whole cells;
- Use isotonic solution to prevent osmotic damage to mitochondria / organelles;
- Keep cold to prevent/reduce damage to organelles by enzyme;
- Use buffer to maintain pH and prevent protein/enzyme denaturation;
- Use differential Centrifuge (at high speed/1000 g) to separate nuclei / cell fragments / heavy organelles;
- Re-spin (supernatant / after nuclei/pellet removed) at higher speed to get mitochondria in pellet/at bottom;
- Observe pellet with a microscope to identify mitochondria;
Describe the structure of a phospholipid molecule and explain how phospholipids are arranged in a plasma membrane (3 marks).
- Glycerol joined to two fatty acid tails Phosphate group joined to glycerol on opposite side. (joined by condensation reaction with ester bond).;
- Phospholipid has hydrophilic head (phosphate and glycerol) and hydrophobic tails (fatty acid chains)
- Arrange to form a phospholipid bilayer; (Hydrophilic head facing out. Hydrophobic fatty acid chains facing in)
Describe the non-specific defence mechanisms the body may launch against pathogens (5 marks)
The process is called phagocytosis – No Mark
1. Pathogen is engulfed by the phagocyte.
2. Engulfed pathogen enters the cytoplasm of
the phagocyte in a vesicle;
3. Lysosomes fuse with vesicle releasing
digestive enzymes;
4. Lysosome enzymes break down the pathogen.
5. Waste materials are ejected from the cell by exocytosis;
Describe how a phagocyte destroys a pathogen present in the blood.
- Engulfs;
- Forming vesicle/phagosome and fuses with lysosome;
- Enzymes digest/hydrolyse;
Give two types of cell, other than pathogens, that can stimulate an immune response.
- (Cells from) other organisms/transplants;
- Abnormal/cancer/tumour (cells);
- (Cells) infected by virus;
When a vaccine is given to a person, it leads to the production of antibodies against a disease-causing organism. Describe how
- Vaccine contains antigen from pathogen;
- Macrophage presents antigen on its surface;
- T (helper) cell with complementary receptor protein binds to antigen;
- T cell stimulates B cell;
- (With) complementary antibody on its surface;
- B cell divides to form clone secreting / producing same antibody;
- B cell secretes large amounts of antibody;
Explain how the humoral response leads to immunity
- B cells specific to the antigen reproduce by mitosis.
- B cells produce plasma and memory cells
- Second infection produces antibodies in larger quantities AND quicker.
Describe and explain the role of antibodies in stimulating phagocytosis.
- Bind to antigen OR Are markers;
- (Antibodies) cause clumping/agglutination OR Attract phagocytes;
Describe the difference between active and passive immunity.
- Active involves memory cells, passive does not;
- Active involves production of antibody by plasma cells/memory cells;
- Passive involves antibody introduced into body from outside/named source;
- Active long term, because antibody produced in response to antigen;
- Passive short term, because antibody (given) is broken down;
- Active (can) take time to develop/work, passive fast acting;
State why some antibodies are referred to as monoclonal
(Antibodies) produced from a single clone of B cells / plasma cells;
OR
(Antibodies) produced from the same B cell / plasma cell;
Tests using monoclonal antibodies are specific. Use your knowledge of protein structure to explain why.
- Specific) primary structure / order of amino acids;
- (Specific) tertiary / 3D structure / shape;
- (So) Only binds to / fits / complementary to one antigen;
Describe the structure of the human immunodeficiency virus (HIV).
- RNA (as genetic material);
- Reverse transcriptase;
- (Protein) capsomeres/capsid;
- (Phospho)lipid (viral) envelope OR Envelope made of membrane;
- Attachment proteins;
Describe how a person infected with HIV will develop AIDS (if untreated) and die of secondary infections.
- High viral load leads to increased destruction of helper T/CD4 cells;
- Less activation of B cells/cytotoxic T cells/phagocytes;
- Less production of plasma cells/antibodies OR (With cytotoxic T cells) less able to kill virus infected cells;
- (More able to) destroy other microbes/pathogens OR (More able to) destroy mutated/cancer cells;
Describe the role of antibodies in producing a positive result in an ELISA test.
- (First) antibody binds/attaches /complementary (in shape) to antigen; 2. (Second) antibody with enzyme attached is added;
- (Second) antibody attaches to antigen;
- (Substrate/solution added) and colour changes;
How does oxygen move through the insect?
- Oxygen diffuses in through the spiracles;
- Spiracle closes;
- Oxygen moves through the trachea into the tracheoles;
- Oxygen delivered directly to the respiring tissues;