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Blood Bank Rotation > IUHPL - Blood Bank - Crossmatch/Automated > Flashcards

IUHPL - Blood Bank - Crossmatch/Automated Flashcards

1
Q

What must be on a patient labile to be acceptable for compatibility testing?

A
  • 2 patient idientifiers
  • date of collection
  • phlebotomist initials
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2
Q

What must a requisition have to be complete

A

*

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3
Q

What BB tests are performed for this request:

- type and screen

A

ABO/Rh, IAT; ABID if IAT is positive

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4
Q

What BB tests are performed for this request:

- crossmatch

A

ABO/Rh, IAT, XM, ABID and full XM if IAT is positive

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5
Q

What BB tests are performed for this request:

- cord blood work-up

A

ABO/Rh (no reverse) DAT

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6
Q

What BB tests are performed for this request:

- RHIG samples

A

ABO/Rh, IAT, FMH RapidScreen; ABID as indicated (no titers)

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7
Q

What BB tests are performed for this request:

- prenatal samples

A

ABO/Rh, IAT; ABID and titer if indicated

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8
Q

What BB tests are performed for this request:

- DAT

A

DAT with polyspecific AHG; equate if positive with IgG coating cells

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9
Q

What BB tests are performed for this request:

- transfusion reactions

A

DAT, visual hemolysis check and clerical check on all; full workup if either DAT or hemolysis check are positive (ABO/Rh, IAT, XM, ABID, Eluate)

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10
Q

What BB tests are performed for this request:

- neonate crossmatch

A

NNP ABO/Rh; Screen; DAT; ABO/screen if A,B or AB

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11
Q

What BB tests are performed for this request:

- antibody identification

A
  • Panel or 3 positive and 3 negative cells to prove the antibody
  • All common alloantibodies must be excluded
  • Antigen type of patient must be neg for the Ab ID’d
  • Antigen type and full XM for each donor (must be neg for Ab ID’d)
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12
Q

What BB tests are performed for this request:

- special antigen typing

A

Antisera (room temp incubation and spin and read or AHG read if IgG) monoclonal don’t incubate

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13
Q

What is the use of an Immediate Spin Crossmatch? Limitations?

A

detects most ABO incompatibilities; can only be used if patient has no histories or current antibodies

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14
Q

What is the use of an Antiglobulin Crossmatch? Limitations?

A

must perform (addition to IS XM) if there is a history of or current unexpected, clinically significant antibody

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15
Q

What is the use of a Computer Crossmatch? Limitations?

A

compares recent ABO serologic results and interpretation on file for both donor and recipient. Current Ab screen must be negative/ no history of Ab/ 2 Rh types must be on file

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16
Q

What are the incompatibilities in Immediate Spin Crossmatches

A

*

17
Q

What are the incompatibilities in Antiglobulin Crossmatches

A

*

18
Q

Solid Phase:

- test menu

A

ABO/Rh; indirect/direct; XM; ABID

19
Q

Solid Phase:

- interpretation of results

A

Pos: agglutination is seen as monolayer or RBCs
Neg: no agglutination - forms button at bottom of well

20
Q

Solid Phase:

- reflex testing

A

*

21
Q

Solid Phase:

- advantages

A

*

22
Q

Solid Phase:

- disadvantages

A
  • can be too sensitive and pick up junk

- must warm antisera

23
Q

Solid Phase:

- QC/maintenance requirements

A

*

24
Q

Gel Methodologies:

- test menu

A

AB screen / DAT/ ABID

25
Q

Gel Methodologies:

- interpretation of results

A

Pos: aggltinated RBCs remain at top or suspended in gel
Neg: unagglutinated RBCs form a pellet at bottom of gel - no cross linking

26
Q

Gel Methodologies:

- reflex testing

A
  • up to interpretation
  • must remember stir balls (or get false pos)
  • a lot of machine maintenance
27
Q

Gel Methodologies:

- advantages

A

*

28
Q

Gel Methodologies:

- disadvantages

A
  • person has to interpret results
  • need to warm reagents
  • can get mixed field
29
Q

Gel Methodologies:

- QC/maintenance requirements

A

Day of use

30
Q

Tube methodologies:

- test menu

A

DATs

31
Q

Tube methodologies:

- interpretation of results

A

Positive: agglutination forms as button
Negative: no agglutination fades into background

32
Q

Tube methodologies:

- reflex testing

A

*

33
Q

Tube methodologies:

- advantages

A

*

34
Q

Tube methodologies:

- disadvantages

A
  • up to interpretation
35
Q

Tube methodologies:

- QC/maintenance

A

reagent rack needs QC daily

Blood Bank Rotation (6 decks)

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