IUHPL - Blood Bank - Crossmatch/Automated Flashcards
What must be on a patient labile to be acceptable for compatibility testing?
- 2 patient idientifiers
- date of collection
- phlebotomist initials
What must a requisition have to be complete
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What BB tests are performed for this request:
- type and screen
ABO/Rh, IAT; ABID if IAT is positive
What BB tests are performed for this request:
- crossmatch
ABO/Rh, IAT, XM, ABID and full XM if IAT is positive
What BB tests are performed for this request:
- cord blood work-up
ABO/Rh (no reverse) DAT
What BB tests are performed for this request:
- RHIG samples
ABO/Rh, IAT, FMH RapidScreen; ABID as indicated (no titers)
What BB tests are performed for this request:
- prenatal samples
ABO/Rh, IAT; ABID and titer if indicated
What BB tests are performed for this request:
- DAT
DAT with polyspecific AHG; equate if positive with IgG coating cells
What BB tests are performed for this request:
- transfusion reactions
DAT, visual hemolysis check and clerical check on all; full workup if either DAT or hemolysis check are positive (ABO/Rh, IAT, XM, ABID, Eluate)
What BB tests are performed for this request:
- neonate crossmatch
NNP ABO/Rh; Screen; DAT; ABO/screen if A,B or AB
What BB tests are performed for this request:
- antibody identification
- Panel or 3 positive and 3 negative cells to prove the antibody
- All common alloantibodies must be excluded
- Antigen type of patient must be neg for the Ab ID’d
- Antigen type and full XM for each donor (must be neg for Ab ID’d)
What BB tests are performed for this request:
- special antigen typing
Antisera (room temp incubation and spin and read or AHG read if IgG) monoclonal don’t incubate
What is the use of an Immediate Spin Crossmatch? Limitations?
detects most ABO incompatibilities; can only be used if patient has no histories or current antibodies
What is the use of an Antiglobulin Crossmatch? Limitations?
must perform (addition to IS XM) if there is a history of or current unexpected, clinically significant antibody
What is the use of a Computer Crossmatch? Limitations?
compares recent ABO serologic results and interpretation on file for both donor and recipient. Current Ab screen must be negative/ no history of Ab/ 2 Rh types must be on file
What are the incompatibilities in Immediate Spin Crossmatches
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What are the incompatibilities in Antiglobulin Crossmatches
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Solid Phase:
- test menu
ABO/Rh; indirect/direct; XM; ABID
Solid Phase:
- interpretation of results
Pos: agglutination is seen as monolayer or RBCs
Neg: no agglutination - forms button at bottom of well
Solid Phase:
- reflex testing
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Solid Phase:
- advantages
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Solid Phase:
- disadvantages
- can be too sensitive and pick up junk
- must warm antisera
Solid Phase:
- QC/maintenance requirements
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Gel Methodologies:
- test menu
AB screen / DAT/ ABID
Gel Methodologies:
- interpretation of results
Pos: aggltinated RBCs remain at top or suspended in gel
Neg: unagglutinated RBCs form a pellet at bottom of gel - no cross linking
Gel Methodologies:
- reflex testing
- up to interpretation
- must remember stir balls (or get false pos)
- a lot of machine maintenance
Gel Methodologies:
- advantages
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Gel Methodologies:
- disadvantages
- person has to interpret results
- need to warm reagents
- can get mixed field
Gel Methodologies:
- QC/maintenance requirements
Day of use
Tube methodologies:
- test menu
DATs
Tube methodologies:
- interpretation of results
Positive: agglutination forms as button
Negative: no agglutination fades into background
Tube methodologies:
- reflex testing
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Tube methodologies:
- advantages
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Tube methodologies:
- disadvantages
- up to interpretation
Tube methodologies:
- QC/maintenance
reagent rack needs QC daily
