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AB Protein Ligand Interactions > Isothermal Titration Calorimetry > Flashcards

Isothermal Titration Calorimetry Flashcards

1
Q

Components ITC

A

Reference cell
Sample cell
Syringe containing titrant (ligand)

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2
Q

Principle ITC

A

Heat of binding protein-ligand
- heat absorbed: endothermic
- heat released: exothermic

Maintainf Delta T bw cells = 0

Quantity of heat(Q) applied to reference cell = amount binding

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3
Q

What you get ITC

A

KD
Stoichiometry of binding (ratio protein: ligand)
Delta H, G, S

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AB Protein Ligand Interactions (4 decks)

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