isolation media

Question 1

Isolation of gram-positive and gram-negative anaerobes

Answer 1

Anaerobic phenlyethyl Alcohol Agar

Question 2

Isolation of gram-negative anaerobes

Answer 2

Anaerobic kanamycin-vancomycin agar

Question 3

Isolation of Bordetella pertussis

Answer 3

Bordet-Gengou media
Regan-Lowe Agar

Question 4

Enrichment used to isolate Legionella

Answer 4

Buffered Charcoal Yeast Extract

Question 5

Isolation of Campylobacter, Brucella agar and sheep blood with antibiotics

Answer 5

Campy Blood Agar

Question 6

Enriched, nonselective medium to isolate fastidious organisms, such as Neisseria and Haemophilus

Answer 6

Chocolate Agar

Question 7

Isolation of Yersinia species

Answer 7

Cefulodin-Irgasan-Novocibin Agar

Question 8

Isolation of gram-positive bacteria

Answer 8

Colistin-nalidixic acid Agar

Question 9

Differential: isolation of gram-negative bacilli and differentiation of lactose fermenters from non-lactose fermenters

Answer 9

Eosin-methylene Blue Agar
MacConkey Agar

Question 10

Selective: isolation of stool pathogens through inhibition of normal flora coliforms

Answer 10

Hektoen Enteric Agar
Salmonella-Shigella Agar
Xylose-lysine-deoxycholate Agar

Question 11

Primary isolation medium for Mycobacterium

Answer 11

Lowenstein-Jensen Agar
Middlebrook Medium
Petragnani’s Medium

Question 12

Isolation of Neisseria gonorrhoeae

Answer 12

Martin-Lewis Medium
Modified Thayer-Martin

Question 13

Enriched, nonselective Medium

Answer 13

Sheep Blood Agar

Question 14

Isolation and differentiation of Vibrio

Answer 14

Thiosulfate-Citrate-bile salts-sucrose Agar

Question 15

All-purpose isolation media; subculture from primary plate

Answer 15

Trypticase Soy Agar

Question 16

Enrichment broth to enhance recovery of stool pathogens and inhibit normal flora coliforms

Answer 16

Gram-negative Broth
Selenite Broth
Tetrathionate Broth

Question 17

Enriched broth that permits growth at various oxygen levels

Answer 17

Thioglycolate Broth