IS Flashcards
1
Q
1000 AD
A
Chinese Practice of Insufflation
2
Q
1798 Smallpox vaccine
Cross Immunity
A
Edward Jenner
3
Q
1862 Phagocytosis
A
Haeckel
4
Q
1865-1866 Mendelian Inheritance
A
Gregor Mendel
5
Q
1880-1881 Live attenuated chicken cholera and anthrax vaccine
A
Louis Pasteur
6
Q
1883-1905 Cellular Theory of Immunity: PHAGOCYTOSIS
A
Metchnikoff
7
Q
1885 Live, attenuated RABIES VACCINE
A
Louis Pasteur
8
Q
1890 Humoral theory of Immunity
A
Emil von Behring, Kitasata
9
Q
1891 Type IV Hypersensitivity Demonstration
A
Robert Koch
10
Q
1900 Antibody Formation Theory
A
Ehrlich
11
Q
1903 Arthus Reaction, Hypersensitivity
A
Arthus
12
Q
1903 Opsonins
A
Almorth Wright
13
Q
1930 C-Reactive Protein
A
Tillet and Francis
14
Q
1894 Complement
A
Jules Bordet
15
Q
1902 Immediate-Anaphylaxis Hypersensitivity
A
Portier, Richet
16
Q
1938 Ag-Ab Binding
A
Marrack
17
Q
1949 Polio vaccine
A
Salk and Sabin
18
Q
1951 Yellow Fever Vaccine
A
Reed
19
Q
1957 CIonal Selection Theory
A
Burnet
20
Q
1975 Monocional Antibodies
A
Georges Kohler, Cesas Milstein
21
Q
1977 Radioimmunassay
A
Rosalyn Yellow
22
Q
T cell Receptor Gene Discovery
A
1935-1987
23
Q
monocional Hepatitis B vaccine Discovery
A
1986
24
Q
1987 Ab Diversity
A
Susumu Tonegawa
25
2005 HPV Vaccine
Frazer
26
Good pasture's Syndrome, multiple sclerosis HLA
DR2
27
SLE, Autoimmune Thyroid Diseases, Dermatitis Herpetiformis
DR3
28
RA HLA present
DR4
29
HLA present in Ankylosing spondylitis (aka Ankyboarthritis Bamboo Spine Disease)
B27 (-90%)
30
HLA present in Celiac Disease, Dermatitis Herpetformis
B8
31
HLA present in Psoriasis Vulgaris
Cw6, B17, B13
32
HLA present in congenital Adrenal Hyperplasia
B47
33
HLA present in Dermatitis Herpetiformis
DR3, B8
34
Test to identify specific HLA Ag
Microlymphocytotoxicity Test
35
Dye: Trypan Blue
Rgt: Battery of Reagent Antisera
Spx: ACD or phenol free Heparinized Blood
AHG: Enhance sensitivity
Microlymphocytotoxicity Test
36
Detection of class I Ag
t-cell
37
Detection of class II Ag
B-cell
38
Quantity the degree of Class II MHC compatibility between potential donors and a recipient
Mixed lymphocyte Reaction
39
Mixed lymphocyte Reaction w/ positive proliferation
Incompatible
40
Mixed Lymphocyte Reaction w/o proliferation
Compatible
41
Test for patient's HLA Ab
LUMINEX (multiple Immunoassay)
42
New method to detect HLA AG
ELISA crossmatch
43
uses Tor B lymphocytes or purified HLA Ag coated onto Microparticles
Flow cytometric Ab screen
44
CRP Normal Concentration
0.47-1.34mg/L
45
<1mg/L CRP
Low Risk CVD
46
1-3mg/L CRP
Average risk for CVD
47
>3mg/L CRP
High Risk CVD
48
Detect future CVD
Hs-CRP
49
Inactivate CRP
Heat serum @ 56°C for 30 mins
50
Serum Amyloid A normal value
~5-8ug/mL
51
What transports SAA to the site of infection?
HDL
52
Normal value for a1-antitrypsin
145-270 mg/dL ( 2-4 g/L)
53
Haptoglobin normal value
40-290 mg/dL
54
Fibrinogen normal value
200-400 mg/dL
55
Ceruloplasmin normal value
20-40 mg/dL
56
Rubor (inc. blood flow and vasodilation)
Erythema/Redness
57
Calor (Inc. blood flow, IL-1B, WBCs
Heat
58
Tumor ( vasodilation, plasma leakage)
Edema
59
Dolor (pressing of nerve endings)
Pacinian curpuscle
Pain
60
Functio laesa (Repeated 4 cardinal signs, Organ death)
loss of function
61
Double Negative stage
no CD4 and CD8
62
Double positive stage
Express both CD4 and CD8
63
Expresses CD4 and CD25
T-regulatory
64
produces IL-I7 and IL-22
th17
65
produce IL-9
TH9-
66
CD markers for immature T-cell
tdt
CD1a, CD2, CD3,
CD5, CD7, CD34
67
What prolong B-cell life span?
Surface IgD
68
Traditional culture medium for human leukocyte
Roswell Park memorial Institute medium
(RPMI 1640)
69
Gold standard for lymphocyte dentification
Flow Cytometry
70
Activates B cell mitogen
LPS
71
Activates T cells mitogen
Concavalin A, phytonemagglutinin
72
Activate both T and B cells Mitogen
pokeweed mitogen
73
Test for T cell identification
E-rosette Test (+) Rose agglutination
74
End product of T cell activation
cytokines
75
Test to B cell identification
Surface lg Detection
(IgD and IgM on the surface of naive B cells )
76
end product of B cell activation
Antibodies
77
Variable Region
Amino Terminal End
78
Constant Region
Carboxy Terminal End
79
Hinge Region
contains Proline (Flexibility)
80
papain target
1st disulfide bond
81
Fragments of Papain
3 Fragments
Fc (+) Fab (+) Fab
82
Target of Pepsin
2nd disulfide bond
83
Fragments of pepsin
2 Fragments
Fc (+) F(ab)2
84
Unit of sedimentation rate of lg
Svedberg (S)
85
Surfactant to hybridoma technology
Polyethylene Glycol
(Sendai virus: Formerly used)
86
Medium for hybridoma technology
Hypoxanthine-Aminopterin-thymidine
(HAT medium)
87
half life of lgG
23 Days
88
IgG Subclasses that can cross placenta
lgG1, IgG4, IgG3
89
IgG subclass most efficient in crossing placenta
lgG1
90
lgG subclass most efficient in complement Fixation
IgG3
91
Ab Isotype most efficient in PRECIPITATION
IgG
92
lg G Subclasses can fix complement
lgG1, IgG2, IgG3
93
lg found on the Surface of NAIVE B-cells
lgM and IgD
94
Formerly a Reagenic Ab, aka Homocytotropic Ab
Most Heat labile
IgE
95
Force holds hydrophobic interaction
Van der Waal's Force
96
Forces holds hydrophilic interaction
lonic bond
hydrogen bond
97
Secondary Ag-Ab Interaction
Precipitation
Agglutination
98
Bechcet's Syndrome
HLA-B5
99
C-TAD-SMCT
Celiac
Type 1DM
Adrenocortical hyperfunction
Dermytomyositis
Sjogren's
Myasthenia gravis
Chronic Active Hepatitis
Thyrotoxicosis
HLA-B8
100
ASAR
Ankylosing Spondylitis
Spondylitis
Anterior uveitis
Retter's disease
HLA-B27
101
Type 1 DM
HLA-BW15
102
Psoriatic arthritis
HLA-BW38
103
Hashimoto's Thyroiditis
Scleroderma
HLA-DR5
104
sm
SC1
105
(BU) a
SC2
106
SC null
SC3
107
Rd
SC4
108
Star
SC5
109
scer
SC6
110
Scan
SC7
111
1962 high prevalence Ag
Sm (SC1)
112
New low prevalent Ag, 1% in northern Europe but higher in Mennonite population
Bua (SC2)
113
Icteric
>25 mg/dL of Bilirubin
114
kernicterus
18-20 mg/dL of Bilirubin reaches the brain
115
ABO Ab are detected at what age?
3-6 months after birth
116
Neutralized by Hydatid cyst
Anti-P1
117
Potentiators
PEG
LISS
20% Albumin
118
More sensitive than LISS and Albumin but not suitable for px w/ high protein levels
PEG
119
Given for those who are at risk for HIV
CUE cards
120
Screening for varicela Zoster Virus
ELISA
121
Confirmatory for VZV
FAMA
122
Reaction enhanced at pH 6.5-acidic
Anti-M
123
Reaction enhanced at pH >6.5-alkaline
Anti-N
124
Cold reacting Ab seen in Multiparous women
Anti-M
125
Cold reacting ab seen in px with dialysis, machine sterilized with formaldehyde
Anti-N
126
Most abundant Ig in the body
IgA
127
Most abundant Ig in the serum
IgG
128
Longest hinge region in IgG
IgG3
129
Significant Ab Implicated with HTR AND HDN
IgG
130
Ratio of IgG:IgA in the serum
6:1
131
Disease associated with GPA
E.coli
132
Disease associated with GPA and GPB
P. falciparum
133
Disease associated with GPA and GPB
P. falciparum
134
Ab binds complement and reacts obńly in the AHG phase, associated with delayed HTR
Jka, Jkb (kidd)
