Ion Transport Flashcards
how is swelling monitored?
light scattering/ decrease in absorbance
what is an ionophore?
reversible binding channel
what is a symport ionophore channel?
allows 2 oppositely charged molecules to enter the cell (KSCN)
what is an antiport ionophore channel?
allows one charged molecule in with the exit of an oppositely charged molecule
how is the osmolarity in medium containing mitochondria made less than inside matrix?
decrease osmolarity of medium
increase osmolarity of matrix
what must be present in order for X to diffuse down a concentration gradient into matrix?
a transporter
what happens when a charged molecule enters matrix?
membrane potential develops
what is electrogenic transport?
the influx of an ion that generates a membrane potential
what is electroneutral transport?
ions of opposite charges enter matrix
what causes greater swelling electrogenic or neutral transport?
electroneutral
what type of transporter is phosphate?
H+ symporter and OH- antiporter
what happens when phosphate allows an influx of H+?
pH gradient prevents any more form entering
what gets rid of pH gradient and enables swelling?
FCCP
what is valinomycin?
uniport transporter for Na4+ and K+
What is the function of rotenone?
inhibits complex I, ETC is not occuring
