Investigating Enzymatic Reactions Required Practical

Question 1

What does amylase catalyse the breakdown?

Answer 1

starch to maltose

Question 2

What do you put a drop of in every well of the spotting tile?

Answer 2

iodine solution

Question 3

What do you use to heat the beaker of water?

What temperature do you heat up the water to?

How should the temperature be throughout the experiment?

Answer 3

• bunsen burner on a heat proof mat with a tripod and gauze
• 35*C
• constant

Question 4

What do you put inside the boiling tube?

Answer 4

• amylase solution and buffer solution of pH 5

- another syringe for 5cm[cubed] of a starch solution

Question 5

What do you do after mixing the solution?

Answer 5

start a stopwatch

Question 6

How do you use continuous sampling to get the results?

Answer 6

use a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well

Question 7

How do you know starch is no longer present?

Answer 7

the iodine solution remains browny-orange

Question 8

How do you calculate the rate of reaction?

Answer 8

rate = 1000
——-
time