Investigating Enzymatic Reactions Flashcards
How can you investigate the effect of pH on enzyme activity (practical)?
1) Put a drop of iodine solution into every well of a spotting tile.
2) Place a Bunsen burner on a heat-proof mat, and a tripod and gauze over the Bunsen burner. Put a beaker of water on top of the tripod and heat the water until it is 35*c - use a thermometer
3) Add a syringe of 1cm cubed of amylase solution and 1cm cubed of buffer solution with a pH of 5 to a boiling tube. Using test tube holders, put the tube into the beaker of water and wait 5 minutes.
4) Use a different syringe of 5cm cubed of starch solution to the tube.
5) Mix the contents and start a stop clock.
6) Use continuous sampling to record how long it takes for amylase to break down all of the starch - use a dropping pipette to take a fresh sample from the boiling tube every 30 second and put a drop into a well.
7) When the iodine solution remains browny-orange starch is no longer present.
8) Repeat the experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down.
What is the equation for rate of reaction in s^-1?
Rate = 1000/time
What is the equation for Rate of reaction in cm^3/s^-1?
Rate=change/time
