Introduction To Microscopes Flashcards
Good at providing contrast between the organisms edge and its interior and the background
Dark-field micrograph
Entire organism is not in focus at once because of its thickness
Bright-field micrograph
Bacteria not visible
Dark-field micrograph
Bacteria are dark
Phase contrast image
Parts of interior and its detail are visible
Phase contrast image
Lens produce a flat field of view
Plan
Clarity of an image
Resolution
Actual measurement of how far apart two points must be for the microscope to view them
Limit of resolution
Measure of lens ability to capture light coming from the specimen and use it to make the image
Numerical aperture
Only the light reflected off the specimen enters the objective
Dark field microscopy
Phase contrast microscopy
Exploit subtle differences in the refractive indices of water and cytoplasmic components to produce contrast
Uses a fluorescent dye that emits fluorescence when illuminated with ultraviolet radiation
Fluorescence microscopy
Used to clean condenser and objective lens
Lens paper