introduction Flashcards

1
Q

1) . bacteria, fungi, plants, animals, etc. all living organisms inhabit earth with all their distinctive features, what do they have in common? (even the framework is different but the raw materials?)
2) .what stands out when chemical analysis of living and non living is carried out?

A

1) . all organisms are made up of same chemicals, i.e. same elements and compounds
2) .in living organisms, the relative abundance of carbon and hydrogen with respect to other elements is higher than in earth’s crust

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2
Q

acid soluble

A

filtrate

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3
Q

what is used for the chemical analysis of tissue?

A

trichloroacetic acid

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4
Q

acid insoluble pool is the filtrate or retentate? contains?

A

retentate, contains proteins, nucleic acids, polysaccharides and lipids

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5
Q

how to isolate and purify a compound?

A

extract the compounds and subject it to separation techniques.

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6
Q

analytical techniques tell us what?

A

give us an idea of the molecular formula and probable structure of the compound.

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7
Q

define biomolecules.

A

all carbon compounds that we get from living tissues

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8
Q

ash technique is used for? describe it.

A

analysing the inorganic elements and compounds present in the living tissue. A small amount of living tissue (a leaf or a leaf, called wet weight, is weighed and dried. All the water evaporates. The remaining material gives dry weight. When this tissue is completely burnt, the carbon compounds are oxidised to gaseous forms like CO2 and water vapour, and removed leaving behind a remnant called ash. This ash contains many inorganic elements like calcium, magnesium, etc.

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9
Q

acid insoluble

A

retentate

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10
Q

which one of the macromolecules isnt a polymer? why is it included?

A

lipids, they were crushed formed insoluble spherical vesicles which aren’t able to pass through the slits

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11
Q

cytoplasmic components and organelles constitute are classified into macro or micro?

A

macro cytoplasmic constituents and organelle are macromolecules while the rest of the cytoplasmic constituents are found in filtrate

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12
Q

macromolecules weight range above 1,000 daltons.

A

macromolecules above 10,000 daltons

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13
Q

secondary metabolites are formed in?

A

plants, fungi and microbes.

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14
Q

most abundant chemical in living body? percentage?

A

water, 70-90%

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15
Q

most abundant element in living body? percentage?

A

oxygen, 65%

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16
Q

2 elements which reduce excitability of nerves and muscles?

A

calcium and magnesium

17
Q

mitochondrial element

A

manganese

18
Q

nitrogenase element

A

molybdenum

19
Q

ATP enzyme element

A

magnesium

20
Q

nitrogen fixation element

A

molybdenum

21
Q

cytochrome oxidase element

A

copper

22
Q

2 elements found in cytochromes

A

cupric and ferric

23
Q

manganese element is present in which organelle?

A

mitochondria

24
Q

magnesium is present in which enzyme

A

ATP enzymes

25
Q

molybdenum is present in which one of nature’s elemental cycle?

A

nitrogen cycle, nitrogen fixation, nitrogenase

26
Q

how is the proton concentration of cellular fluids maintained?

A

through a an acid-base buffer maintained with the help of dibasic phosphate and monobasic phosphate, both in cells and extracellular fluids.

27
Q

what is the concentration of cations inside the cell?

A

K > Na > Ca