Introduction Flashcards
Storage of fat-soluble vitamins
Fat soluble vitamins A, E, D, and K may all be stored.
Storage of water-soluble vitamins
Water-soluble vitamins are not stored, they are excreted constantly by the kidneys. Thus, you must take in a constant stream of water-soluble vitamins A and the various B vitamins
Rate Constant Equation
Transition state thermodynamics
Catalysis Thermodynamics
Why is it better for the enzyme to conform to the transition state rather than to the substrate?
Lineweaver-Burke Plot
Inhibitor Flow Chart
If the inhibitor does not covalently modify the enzyme, it is then classified as a reversible inhibitor. Because these inhibitors can rapidly bind and dissociate from the enzyme, we can further analyze the rela5onship between inhibitor binding and substrate binding. If I (inhibitor) and S (substrate) cannot bind the enzyme at the same time, this is referred to as competitive inhibiton. The opposite of this case is when I can bind to the enzyme ONLY if S is bound first, which is referred to as uncompetitive inhibiton. In between these extremes, binding of S and I may be independent of one another. This is a special case referred to as noncompetitive inhibiton. In reality, pure noncompetitive inhibi5on is fairly rare, as typically the binding of S and I tend to influence one another. Therefore a mixed patern of inhibiton is seen more commonly.
Uses of isoflurophate (a.k.a. diisopropyl fluorophosphate)
Used exclusively to treat glaucoma as a topical eye drop
Dangerous due to its irreversible inhibition of acetylcholinesterase.
Methotrexate
Noncompetitive inhibitors are by definition. . .
Allosteric
Uncompetitive inhibition, the rarest form of inhibition, is usually observed in. . .
enzymes that bind two substrates in a particular order, with the inhibitor resembling one of the two substrates.
Mixed Inhibition
Michaelis-Menten
Michaelis-Menten Derivation
Acetylcholinesterase mechanism
Neuromuscular Junction
Catalytic Triad in AChE
