Introduction Flashcards
1
Q
State 5 points that sum up PCR
A
- PCR is a technique by which any sequence of DNA can be cloned or amplified
- There are no need for cloning vectors, or transformation of bacterial cells followed by screening programme
- DNA is amplified entirely in vitro, in an automated system involving a thermocycler
- PCR is rapid and selective that can produce billions of coies of DNA sequences in a few hours.
- This is much faster than the time taken for transformation of bacteria.
2
Q
What process can be used to analyse DNA and PCR products?
A
Gel Electrophoresis
3
Q
How can gene technology be used?
A
- Medicine - pharmaceutical products and diagnosis of disease
- Gene therapy
- Animal cloning
- Transgenic animals/plants
- DNA sequencing
- Pharmacogenetics
- DNA fingerprinting
4
Q
What is DNA fingerprinting?
A
Uses DNA to be able to distinguish between and/or individuals
5
Q
When would DNA fingerprinting be used?
A
- Paternity cases
- Victim identification
- Identifying suspects
6
Q
When was Dolly born?
A
1996
7
Q
Dolly was the first what?
A
The first mammal to be cloned from an adult somatic cell
8
Q
Who was Dolly cloned by?
A
Scientists at Roslin Institute, Edinburgh
9
Q
Where was the donor cell taken from?
A
Taken from white sheep
10
Q
Who was the surrogate mother?
A
Black faced sheep
11
Q
What is a good example of a GM crop?
A
Golden Rice
12
Q
What does it contain?
A
Beta-carotene
