INTRODUCTION Flashcards
Give the Genus of smallest and largest bacteria
Smallest: Mycoplasma
Largest: Bacillus
Antiphagocytic part of bacterial cell wall
Capsule
What bacteria has:
- Much granules
- Babes Ernest/Volutin
- Halberstaedter Prowazek
- Bipolar bodies
- M. tb
- C. diphtheriae
- C. trachomatis
- Y. pestis
Safety pin appearance
Y. pestis
Stain for bipolar bodies of Y. pestis
Wayson’s stain
Presumptive test for L. monocytogenes to demonstate flagella
Hanging drop method
Endotoxin/Exptoxin
- Present in (gm+/-)
- Manner of release
- Chemical component
- Heat stabiliy
Endo
- gm neg
- cell death
- LPS
- heat stable
Exo
- both gm pos and neg
- metabolic product
- protein
- heat labile
Motility:
- Shooting star/Darting
- Tumbling
- Twitching
- Darting
- Gliding/Sliding
- Corkscrew
- Swarming
- Vibrio cholerae
- Listeria monocytogenes
- Kingella kingae
- Campylobacter spp.
- Capnocytophaga gingivalis/Mycoplasma
- Spirochetes
- Proteus spp.
What do you call the organ for locomotion of spirochetes?
Axial filament/Periplasmic flagella
How is cell wall a basis of gram staining?
Thicker peptidoglycan layer = gm (+)
Bacteria that cannot be stained
Cellwalless:
- Genus Mycoplasma
- Genus Ureaplasma
Intracellular
- Rickettsiae
- Chlamydia
Gm neg cell wall have ___ (unique to gm neg), but dont have ___
have: Outer membrane
dont have: Teichoic acids
Gm neg cell wall have ___ (unique to gm neg), but dont have ___
have: Outer membrane
dont have: Teichoic acids
Oxygen requirement (%) of obligate aerobes?
15-21%
Gaspak jar contains __ that will prevent O2
Palladium catalyst
All cocci are gm pos except
Neisseria
Veilonella
Morganella (Branhamella)
All bacilli are gm neg except
Mycobacterium
Clostridium
Corynebacerium
Bacilli
Erysipelothrix
Listeria
Lactobacillus
gm pos or neg
- spiral organisms
gm neg
gm pos or neg
- actinomyces, streptomyces, yeast, and mold
gm pos
acid fast organisms are difficult to stain because of
mycolic acid or hydroxymethoxy acid
All bacteria are non-acid fast except ___; Genus ___ are slightly acid fast
Mycobacterium; Genus Nocardia