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Histology > Introduction > Flashcards

Introduction Flashcards

1
Q

A type of tissue that provides protection, and functions for absorption and secretion

A

Epithelial Tissue

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2
Q

o After the specimen is hardened, it is trimmed into appropriately sized blocks.

o The block is then mounted in microtome and cut with a steel knife.

A

Cutting and Sectioning

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3
Q

Sectioning is done with the aid of

A

Microtome

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4
Q

True or False:

Epithelial Tissues are AVASCULAR.

A

True (They have no Blood Vessels)

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5
Q

is a group of cells WITH INTERRELATED FUNCTION

A

Tissue

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6
Q

Tissues are not always tightly packed with cells but rather is also composed of

A

Fibers and Ground substances.

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7
Q

Fibers and Ground substances are referred to as

A

EXTRACELLULAR MATRIX

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8
Q

o To preserve and support a stained section for light microscopy, it is mounted on a clear glass slide, and covered with a thin glass coverslip

o Placing cut sections on a slide with mounting media such as glycerin or resins

A

Mounting

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9
Q
  • Method is rapid
  • Routinely done in hospital to study specimens during surgery
  • Lipids and enzymes are best preserved in this method
A

Frozen Sections

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10
Q
  • Only done in specimens such as bone and calcified tissues
  • Nitric acid
A

Decalcification

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11
Q

Is removal of excess paraffin

A

Cutting

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12
Q

This Process:

(1) Avoids tissue destruction by digestive enzymes (autolysis) or through bacterial degradation.

(2) Terminate cell metabolism
●Glycolysis without oxygen = lactic acid

(3) Hardens the tissue by cross-linking or denaturing proteins

(4) Kill pathogenic microorganisms such as bacteria, fungi and viruses

A

Fixation

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13
Q

WHY SHOULD IT BE ASCENDING GRADES OF ALCOHOL?

A

TO PRESERVE THE MORPHOLOGY AND APPERANCE OF THE ORGAN.

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14
Q
  • Most commonly used stain in Histology:
A

Hematoxylin and Eosin

o These dyes are water soluble

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15
Q

*Done by successively bathing the specimen in mixture of ethanol and water from 70% to 100%. (increasing concentration of alcohol)

A

Dehydration

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16
Q

This type of tissue provides sensation, control and information processing.

A

Nervous Tissue

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17
Q

Cells + Extracellular Matrix

A

Tissue

18
Q

o Are tissues with negative charges/acids are readily stained with basic dyes

Ex. Nucleic acids = nucleus

A

BASOPHILIC

19
Q
  • The tissue and paraffin will harden after removal from oven
A

Embedding

20
Q
  • After the clearing procedure, the tissue is placed in a melted paraffin in an oven set at 52-60 degree Celsius.
  • The heat causes the clearing agent to evaporate so that the tissue will be filled up with the paraffin
A

INFILTRATION and Embedding

21
Q

Commonly used for Clearing

A

Xylene and Toluene

22
Q

-acidic dye - usually cytoplasmic components and collagen

A

Eosin

23
Q

This type of tissue provides MOVEMENT.

A

Muscle Tissue

24
Q

What are the 4 Basic types of Tissue?

A

Epithelial Tissue
Connective Tissue
Muscle Tissue
Nervous Tissue

25
Q

Is the study of tissues, their functions and arrangement to constitute an organ.

A

HISTOLOGY

26
Q

o Are Tissues with positive charges are stained with acidic dyes

Ex. Mitochondria, collagen, cytoplasm

A

ACIDOPHILIC

27
Q

Keyword of Fixation

A

(TO PRESERVE)

28
Q

The Extracellular Matrix is composed of many kinds of:

A

(1) Ground substances
(2) Fibers

29
Q
  • Since paraffin is colorless, __________ is a must.
  • Application of color to the tissue to highlight structures.
A

Staining

30
Q

What is the Organization of the Human Body (Order)

A

Cells
Tissue
Organ
System

31
Q
  • Removal of the dehydrating agent by immersing the specimen in the solvent that the alcohol and embedding medium is miscible
  • Clearing agent are highly volatile (becomes vapor once exposed to heat)
A

Clearing

32
Q

-basic dye; usually stains nucleus and RNA-containing portion of cytoplasm

A

Hematoxylin

33
Q

True or False:

Decalcification is NOT only limited to bones and teeth.

A

True

34
Q

Branch of Anatomy = also known as Microscopic anatomy

A

HISTOLOGY

35
Q

Used to remove water out of tissue.

A

Alcohol

36
Q

The faster way to do Biopsy

A

FROZEN SECTION BIOPSY

37
Q

This type of tissue is highly vascularized, to which it shares blood supply and oxygen to the tightly packed epithelial tissue.

A

Connective Tissue

38
Q

Commonly used fixative

A

Formalin

39
Q

STANDARD ROUTINE TISSUE PROCESSING:

A
  • FIXATION
  • DECALCIFICATION - IF CALCIFIED SPX
  • DEHYDRATION
  • CLEARING
  • INFILTRATION
  • EMBEDDING
  • SECTIONING
  • STAINING
  • MOUNTING
  • TURN AROUND TIME IS 5 DAYS.
40
Q

Since cellular decomposition begins immediately after the death of a human/patient, tissues must be _______ to prevent alterations in their structure through decomposition.

A

Fixed

41
Q

are responsible for connecting the epithelial tissues to the muscle tissue

A

Connective Tissue

42
Q

True or False:

Cells in the tissue need to be the same type, or same morphology.

A

False

Histology (2 decks)

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