Introduction

Question 1

A type of tissue that provides protection, and functions for absorption and secretion

Answer 1

Epithelial Tissue

Question 2

o After the specimen is hardened, it is trimmed into appropriately sized blocks.

o The block is then mounted in microtome and cut with a steel knife.

Answer 2

Cutting and Sectioning

Question 3

Sectioning is done with the aid of

Answer 3

Microtome

Question 4

True or False:

Epithelial Tissues are AVASCULAR.

Answer 4

True (They have no Blood Vessels)

Question 5

is a group of cells WITH INTERRELATED FUNCTION

Answer 5

Tissue

Question 6

Tissues are not always tightly packed with cells but rather is also composed of

Answer 6

Fibers and Ground substances.

Question 7

Fibers and Ground substances are referred to as

Answer 7

EXTRACELLULAR MATRIX

Question 8

o To preserve and support a stained section for light microscopy, it is mounted on a clear glass slide, and covered with a thin glass coverslip

o Placing cut sections on a slide with mounting media such as glycerin or resins

Answer 8

Mounting

Question 9

• Method is rapid
• Routinely done in hospital to study specimens during surgery
• Lipids and enzymes are best preserved in this method

Answer 9

Frozen Sections

Question 10

• Only done in specimens such as bone and calcified tissues
• Nitric acid

Answer 10

Decalcification

Question 11

Is removal of excess paraffin

Answer 11

Cutting

Question 12

This Process:

(1) Avoids tissue destruction by digestive enzymes (autolysis) or through bacterial degradation.

(2) Terminate cell metabolism
●Glycolysis without oxygen = lactic acid

(3) Hardens the tissue by cross-linking or denaturing proteins

(4) Kill pathogenic microorganisms such as bacteria, fungi and viruses

Answer 12

Fixation

Question 13

WHY SHOULD IT BE ASCENDING GRADES OF ALCOHOL?

Answer 13

TO PRESERVE THE MORPHOLOGY AND APPERANCE OF THE ORGAN.

Question 14

• Most commonly used stain in Histology:

Answer 14

Hematoxylin and Eosin

o These dyes are water soluble

Question 15

*Done by successively bathing the specimen in mixture of ethanol and water from 70% to 100%. (increasing concentration of alcohol)

Answer 15

Dehydration

Question 16

This type of tissue provides sensation, control and information processing.

Answer 16

Nervous Tissue

Question 17

Cells + Extracellular Matrix

Answer 17

Tissue

Question 18

o Are tissues with negative charges/acids are readily stained with basic dyes

Ex. Nucleic acids = nucleus

Answer 18

BASOPHILIC

Question 19

• The tissue and paraffin will harden after removal from oven

Answer 19

Embedding

Question 20

• After the clearing procedure, the tissue is placed in a melted paraffin in an oven set at 52-60 degree Celsius.
• The heat causes the clearing agent to evaporate so that the tissue will be filled up with the paraffin

Answer 20

INFILTRATION and Embedding

Question 21

Commonly used for Clearing

Answer 21

Xylene and Toluene

Question 22

-acidic dye - usually cytoplasmic components and collagen

Answer 22

Eosin

Question 23

This type of tissue provides MOVEMENT.

Answer 23

Muscle Tissue

Question 24

What are the 4 Basic types of Tissue?

Answer 24

Epithelial Tissue
Connective Tissue
Muscle Tissue
Nervous Tissue

Question 25

Is the study of tissues, their functions and arrangement to constitute an organ.

Answer 25

HISTOLOGY

Question 26

o Are Tissues with positive charges are stained with acidic dyes Ex. Mitochondria, collagen, cytoplasm

Answer 26

ACIDOPHILIC

Question 27

Keyword of Fixation

Answer 27

(TO PRESERVE)

Question 28

The Extracellular Matrix is composed of many kinds of:

Answer 28

(1) Ground substances (2) Fibers

Question 29

* Since paraffin is colorless, __________ is a must. * Application of color to the tissue to highlight structures.

Answer 29

Staining

Question 30

What is the Organization of the Human Body (Order)

Answer 30

Cells Tissue Organ System

Question 31

* Removal of the dehydrating agent by immersing the specimen in the solvent that the alcohol and embedding medium is miscible * Clearing agent are highly volatile (becomes vapor once exposed to heat)

Answer 31

Clearing

Question 32

-basic dye; usually stains nucleus and RNA-containing portion of cytoplasm

Answer 32

Hematoxylin

Question 33

True or False: Decalcification is NOT only limited to bones and teeth.

Answer 33

True

Question 34

Branch of Anatomy = also known as Microscopic anatomy

Answer 34

HISTOLOGY

Question 35

Used to remove water out of tissue.

Answer 35

Alcohol

Question 36

The faster way to do Biopsy

Answer 36

FROZEN SECTION BIOPSY

Question 37

This type of tissue is highly vascularized, to which it shares blood supply and oxygen to the tightly packed epithelial tissue.

Answer 37

Connective Tissue

Question 38

Commonly used fixative

Answer 38

Formalin

Question 39

STANDARD ROUTINE TISSUE PROCESSING:

Answer 39

* FIXATION * DECALCIFICATION - IF CALCIFIED SPX * DEHYDRATION * CLEARING * INFILTRATION * EMBEDDING * SECTIONING * STAINING * MOUNTING * TURN AROUND TIME IS 5 DAYS.

Question 40

Since cellular decomposition begins immediately after the death of a human/patient, tissues must be _______ to prevent alterations in their structure through decomposition.

Answer 40

Fixed

Question 41

are responsible for connecting the epithelial tissues to the muscle tissue

Answer 41

Connective Tissue

Question 42

True or False: Cells in the tissue need to be the same type, or same morphology.

Answer 42

False