INTRODUCTION Flashcards
Selected tissue specimen is immersed in a watchglass with isotonic solutions, dissected, separated and examined under the microscope
Teasing or Dissociation
Small pieces of tissue not more than 1mm in the microscopic slide and forcibly compressed with another slide or coverslip
Squash Preparation (Crushing)
Cellular materials are spread lighlty by a wire loop or applicator, or by making an apposition smear with another slide
Smear Preparation
Useful in cytological examination particular cancer diagnosis
Smear Preparation
Normally utilized for rapid diagnosis of tissue
Frozen section
This method is recommended for lipids and nervous tissue elements
Frozen section
Non fatty unfixed tissues are sectioned well temperatures between
-10’ and -25’ C
Most rapid and commonly available freezing agents that is generally used in histochemistry and operative procedures
Liquid nitrogen