Intro to Histology Flashcards

1
Q

What is the most common stain?

a) which stains for acidic structures like nuclei (basic dye)
b) which stains for proteins like cytoplasm (acidic dye)

A

1.Hematoxylin
A basic dye
Stains strictures containing acids (e.g. nuclei) blue (arrow)

  1. Eosin
    An acidic dye
    Stains structures containing protein (e.g cytoplasm) pink (square)
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2
Q

What is the following stain:

  • Stains Bacteria
  • background is yellow
  • positive is PURPLE (peptidoglycan)
A
  1. Gram stain
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3
Q

What is the following stain:

Stains Fungi, Glycogen

  • background is light pink
  • positive is BRIGHT pink (magenta)
A

Periodic acid–Schiff (PAS)

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4
Q

What is the following stain:

–Stains fungi

  • background green
  • positive = BLACK
A

Grocott methenamine silver stain (GMS)

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5
Q

What is the following stain:

-Stains - Mycobacteria, Actinomyces (background blue / positive red)

A

Acid Fast

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6
Q

What stain is used for antibodies?

A

IHC

positive = BROWN/RED

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7
Q

State the following for skeletal, cardiac & smooth muscle

  1. Striations
  2. Nuclei
  3. Intercalated disks
A

SKELETAL:

  1. Yes striations
  2. Multiple Nuclei
  3. No disks

Cardiac:

  1. Yes striations
  2. Single Nuclei
  3. YES disks

SMOOTH:

  1. NO!! striations
  2. Single Nuclei
  3. No disks
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8
Q

______ tissue is made of fibroblasts with nuclei that are cigar shaped and evenly distributed.

A

CONNECTIVE tissue

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9
Q

The following describes what type of tissue:

  1. No defined boundary
  2. Linear Eosinophilic
  3. Indistinct cell border
  4. Uniform cytoplasm
  5. Cigar shaped nuclei
  6. Evenly distributed nuclei
A

CONNECTIVE TISSUE

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10
Q

The following describes what type of tissue:

  1. Fasciles w/ perineum
  2. WAVY Eosinophilic
  3. DISTINCT cell border
  4. Uniform cytoplasm
  5. FOAMY shaped nuclei
  6. nuclei in CENTER of cell
A

Nervous tissue

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11
Q

The following describes what type of tissue:

  1. Bundles w/ perimysium
  2. WAVY Eosinophilic
  3. DISTINCT cell border
  4. Uniform cytoplasm
  5. CIGAR shaped nuclei
  6. nuclei in CENTER of cell
A

Smooth Muscle

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12
Q

The following describes what type of tissue:

  1. Bundles w/ perimysium
  2. LINEAR Eosinophilic
  3. DISTINCT cell border
  4. STRIATED cytoplasm
  5. CIGAR shaped nuclei
  6. nuclei in periphery/center
A

SKELETAL/ cardiac muscle

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13
Q

Chronic inflammation lacks ______

A

NEUTROPHILS

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14
Q

____ are multi lobed nuclei

and ____ are bilobed

(eosinophilic / neutrophil)

A
  1. Neutrophil = multi

2. Eosinophil = bi

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