Intro to Histology Flashcards
What is the most common stain?
a) which stains for acidic structures like nuclei (basic dye)
b) which stains for proteins like cytoplasm (acidic dye)
1.Hematoxylin
A basic dye
Stains strictures containing acids (e.g. nuclei) blue (arrow)
- Eosin
An acidic dye
Stains structures containing protein (e.g cytoplasm) pink (square)
What is the following stain:
- Stains Bacteria
- background is yellow
- positive is PURPLE (peptidoglycan)
- Gram stain
What is the following stain:
Stains Fungi, Glycogen
- background is light pink
- positive is BRIGHT pink (magenta)
Periodic acid–Schiff (PAS)
What is the following stain:
–Stains fungi
- background green
- positive = BLACK
Grocott methenamine silver stain (GMS)
What is the following stain:
-Stains - Mycobacteria, Actinomyces (background blue / positive red)
Acid Fast
What stain is used for antibodies?
IHC
positive = BROWN/RED
State the following for skeletal, cardiac & smooth muscle
- Striations
- Nuclei
- Intercalated disks
SKELETAL:
- Yes striations
- Multiple Nuclei
- No disks
Cardiac:
- Yes striations
- Single Nuclei
- YES disks
SMOOTH:
- NO!! striations
- Single Nuclei
- No disks
______ tissue is made of fibroblasts with nuclei that are cigar shaped and evenly distributed.
CONNECTIVE tissue
The following describes what type of tissue:
- No defined boundary
- Linear Eosinophilic
- Indistinct cell border
- Uniform cytoplasm
- Cigar shaped nuclei
- Evenly distributed nuclei
CONNECTIVE TISSUE
The following describes what type of tissue:
- Fasciles w/ perineum
- WAVY Eosinophilic
- DISTINCT cell border
- Uniform cytoplasm
- FOAMY shaped nuclei
- nuclei in CENTER of cell
Nervous tissue
The following describes what type of tissue:
- Bundles w/ perimysium
- WAVY Eosinophilic
- DISTINCT cell border
- Uniform cytoplasm
- CIGAR shaped nuclei
- nuclei in CENTER of cell
Smooth Muscle
The following describes what type of tissue:
- Bundles w/ perimysium
- LINEAR Eosinophilic
- DISTINCT cell border
- STRIATED cytoplasm
- CIGAR shaped nuclei
- nuclei in periphery/center
SKELETAL/ cardiac muscle
Chronic inflammation lacks ______
NEUTROPHILS
____ are multi lobed nuclei
and ____ are bilobed
(eosinophilic / neutrophil)
- Neutrophil = multi
2. Eosinophil = bi