Intro To Histo Flashcards
Magnification
Ability to produce enlarged images
Resolution
Ability to visualize small particles that are close together as separate objects
Resolution Depends on
Wavelength of light source
Specimen thickness
Quality of fixation
Staining intensity
Light Microscope
Can visualize tissues
Uses light and glass lenses
TEM
Electron beam passes through thin tissue section of a specimen to create image
Can see organelles
SEM
Electron beam is scanned across specimen to create 3D images
Can see close up of organelles (i.e. ribosomes)
Hematoxylin
Positively charged
Stains negatively charged (basophilic) components blue
Eosin
Negatively charged
Stains positively charged (eosinophilic) components pink
PAS
Stains glycogen and CHO
Once in
Stains elastic fibers dark brown
Stains mast cells purple
Stains smooth muscle light blue
Silver
Stains reticular fibers and nerve fibers brown/black
Romanowsky Staining
Used in blood films
Stains nucleus of WBC dark blue/purple
Decalcification
Removal of mineral from bone and other hard tissue prior to tissue processing
Acid or cheating agents used
Ground Section
Section is ground down with abrasive material until hard tissue is thin enough to transmit light
Ca salts are not removed
Types of artifacts
Forceps/crush Heat co-agulation Tear Fixation Freezing
