Intro To Biotechnology Flashcards

1
Q

What are the 4 major domains of Biotechnology?

A

~Industrial and Environmental Biotechnology
~Medical/Pharmaceutical Biotechnology
~Agricultural Biotechnology
~Diagnostic Research Biotechnology

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2
Q

What does SLOP mean?

A

Standard Laboratory Operating Procedures

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3
Q

Two reasons why it is important to maintain SLNB

A

~It becomes the historical record of all work.

~It may be required in court cases.

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4
Q

How do you fix incorrect entries in the SLNB?

A

You mark through it a single time and label in with your initials and the date.

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5
Q

How are pages in SLNB signed and witnessed?

A

~They are signed and dated at the bottom of the page.

~Also must be signed by someone who is not your lab partner.

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6
Q

What does REE mean?

A

Results with Evidence and Explanation

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7
Q

What does PE mean?

A

Possible Errors

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8
Q

What does PA mean?

A

Practical Applications

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9
Q

How far does biotechnology date back?

A

6000 B.C.

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10
Q

Why does biotech companies usually specialize in groups of similar products?

A
~similar manufacturing process
~same equipment 
~same reagents 
~more economical 
~saves time in research and development
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11
Q

What are the steps of a product pipeline?

A
~Product identification 
~Research and development 
~Small scale manufacturing 
~Testing the safety and efficacy 
~Manufacturing 
~Sales and marketing
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12
Q

What is the difference between large and small scale testing?

A

Small scale testing is more answering questions about the product and large scale testing is clinical trails under the FDA.

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13
Q

What is a clinical trial?

A

It is a series of testing that is done on pharmaceuticals and procedures. First the product is tested on a small group and the group gradually get larger as the process goes on. These tests are overseen by the FDA and take 10-15 years to complete.

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14
Q

What are 3 government agencies that monitor trials and production of biotech products.(Acronym and full name)

A

FDA~ Food and Drug Administration
EPA~Environmental Protection Agency
USDA~ United States Department of Agriculture

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15
Q

A _________ pipette is used to deliver a single specific volume of liquid.

A

Volumetric

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16
Q

A _________ pipette is calibrated into small divisions so that varying amounts of liquid can be measured.

A

Serological

17
Q

Which pipette is more accurate?

A

Volumetric

18
Q

What does TD printed on the neck of a pipette mean?

A

To deliver

19
Q

What does TC printed on the neck of a pipette mean?

A

To contain

20
Q

Which pipette is a blow out pipette?

A

Serological

21
Q

How do you read a meniscus?

A

You take the measurement of the bottom of the line.

22
Q

DNA

A

Deoxyribonucleic Acid

23
Q

Recombinant DNA

A

When you take genetic material from two organisms and combine them.

24
Q

What is the variable in an experiment?

A

It is the part of the experiment that is caused by reaction.

25
Q

What is the control in an experiment?

A

It is the part of the experiment that the scientist can control

26
Q

Defined as any technique that uses-_____________ or their products to make or modify a product, to improve ____________, or to develop _______ for specific uses.

A

Living organisms
Plants or Animal
Microorganism

27
Q

Things to avoid when using a micro pipettes

A

~Never use micro pipettes without the tip

~Keep pipettes vertical at all times

28
Q

What do the two stops on a micro pipette mean?

A

1st~ To get the set volume

2nd~ Removes full volume, evacuating the tip

29
Q

What has biotechnology revolutionized the development of?

A

Creating recombinant DNA

30
Q

What does recombinant DNA create

A

New combos of DNA

31
Q

What was the first genetically engineered products?

A

Tissue plasminogen activator or t.PA
Scientists genetically modified CHO cells
Blood clot dissolving enzyme

32
Q

First GMO to create human proteins

A

Esherchia coli or E. coli

33
Q

USDA

A

United States Department of Agriculture
Regulates use and production of plants and animals
Decide which animal is acceptable to clone

34
Q

FDA

A

Federal Drug Administration

Regulate use and production of food, feed, veterinary and animal drugs

35
Q

EPA

A

Environmental Protection Agency

Regulate use of microbial agents

36
Q

Strategy for classifying issues in bioethics

A
~ID  and understand issue
~List the solutions to the issue
~ID pros and cons
~Rank all solutions 
~Decide your position and be prepared to describe and defend it
37
Q

Define Biotechnology

A

The study and manipulation of microorganisms for the production of antibiotics, hormones, etc.

38
Q

What does GMO stand for?

A

Genetically Modified Organisms