Intro To Biotechnology

Question 1

What are the 4 major domains of Biotechnology?

Answer 1

~Industrial and Environmental Biotechnology
~Medical/Pharmaceutical Biotechnology
~Agricultural Biotechnology
~Diagnostic Research Biotechnology

Question 2

What does SLOP mean?

Answer 2

Standard Laboratory Operating Procedures

Question 3

Two reasons why it is important to maintain SLNB

Answer 3

~It becomes the historical record of all work.

~It may be required in court cases.

Question 4

How do you fix incorrect entries in the SLNB?

Answer 4

You mark through it a single time and label in with your initials and the date.

Question 5

How are pages in SLNB signed and witnessed?

Answer 5

~They are signed and dated at the bottom of the page.

~Also must be signed by someone who is not your lab partner.

Question 6

What does REE mean?

Answer 6

Results with Evidence and Explanation

Question 7

What does PE mean?

Answer 7

Possible Errors

Question 8

What does PA mean?

Answer 8

Practical Applications

Question 9

How far does biotechnology date back?

Answer 9

6000 B.C.

Question 10

Why does biotech companies usually specialize in groups of similar products?

Answer 10

~similar manufacturing process
~same equipment 
~same reagents 
~more economical 
~saves time in research and development

Question 11

What are the steps of a product pipeline?

Answer 11

~Product identification 
~Research and development 
~Small scale manufacturing 
~Testing the safety and efficacy 
~Manufacturing 
~Sales and marketing

Question 12

What is the difference between large and small scale testing?

Answer 12

Small scale testing is more answering questions about the product and large scale testing is clinical trails under the FDA.

Question 13

What is a clinical trial?

Answer 13

It is a series of testing that is done on pharmaceuticals and procedures. First the product is tested on a small group and the group gradually get larger as the process goes on. These tests are overseen by the FDA and take 10-15 years to complete.

Question 14

What are 3 government agencies that monitor trials and production of biotech products.(Acronym and full name)

Answer 14

FDA~ Food and Drug Administration
EPA~Environmental Protection Agency
USDA~ United States Department of Agriculture

Question 15

A _________ pipette is used to deliver a single specific volume of liquid.

Answer 15

Volumetric

Question 16

A _________ pipette is calibrated into small divisions so that varying amounts of liquid can be measured.

Answer 16

Serological

Question 17

Which pipette is more accurate?

Answer 17

Volumetric

Question 18

What does TD printed on the neck of a pipette mean?

Answer 18

To deliver

Question 19

What does TC printed on the neck of a pipette mean?

Answer 19

To contain

Question 20

Which pipette is a blow out pipette?

Answer 20

Serological

Question 21

How do you read a meniscus?

Answer 21

You take the measurement of the bottom of the line.

Question 22

DNA

Answer 22

Deoxyribonucleic Acid

Question 23

Recombinant DNA

Answer 23

When you take genetic material from two organisms and combine them.

Question 24

What is the variable in an experiment?

Answer 24

It is the part of the experiment that is caused by reaction.

Question 25

What is the control in an experiment?

Answer 25

It is the part of the experiment that the scientist can control

Question 26

Defined as any technique that uses-_____________ or their products to make or modify a product, to improve ____________, or to develop _______ for specific uses.

Answer 26

Living organisms Plants or Animal Microorganism

Question 27

Things to avoid when using a micro pipettes

Answer 27

~Never use micro pipettes without the tip | ~Keep pipettes vertical at all times

Question 28

What do the two stops on a micro pipette mean?

Answer 28

1st~ To get the set volume | 2nd~ Removes full volume, evacuating the tip

Question 29

What has biotechnology revolutionized the development of?

Answer 29

Creating recombinant DNA

Question 30

What does recombinant DNA create

Answer 30

New combos of DNA

Question 31

What was the first genetically engineered products?

Answer 31

Tissue plasminogen activator or t.PA Scientists genetically modified CHO cells Blood clot dissolving enzyme

Question 32

First GMO to create human proteins

Answer 32

Esherchia coli or E. coli

Question 33

USDA

Answer 33

United States Department of Agriculture Regulates use and production of plants and animals Decide which animal is acceptable to clone

Question 34

FDA

Answer 34

Federal Drug Administration | Regulate use and production of food, feed, veterinary and animal drugs

Question 35

EPA

Answer 35

Environmental Protection Agency | Regulate use of microbial agents

Question 36

Strategy for classifying issues in bioethics

Answer 36

``` ~ID and understand issue ~List the solutions to the issue ~ID pros and cons ~Rank all solutions ~Decide your position and be prepared to describe and defend it ```

Question 37

Define Biotechnology

Answer 37

The study and manipulation of microorganisms for the production of antibiotics, hormones, etc.

Question 38

What does GMO stand for?

Answer 38

Genetically Modified Organisms